159,461 results

1. LbCas12a-niCPE

   Plasmid

   #213747

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-nLbCas12a-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, R1138A in LbCas12a

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUBQ:Citrine-TurboID-3xMyc

   Plasmid

   #199244

   Purpose

   This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

   Insert

   Citrine-TurboID-3xMyc
   

   Use

   Tags

   TurboID-3xMyc

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis UBQ10 promoter with intron

   Available since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-PLXNA3

   Plasmid

   #23518

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PLXNA3 (PLXNA3 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   L1655M

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. NLS-Halo

   Plasmid

   #200197

   Purpose

   Expression for SV40 NLS

   Depositor

   Sheila Teves

   Article

   Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

   Insert

   3X SV40 NLS-Halo
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   None

   Promoter

   EF_1alpha

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PJEBAN6

   Plasmid

   #203684

   Purpose

   Expresses red fluorescent marker DsRed-Express in Listeria monocytogenes

   Depositor

   Tine Rask Licht

   Article

   Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

   Insert

   DsRed-Express
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

   Plasmid

   #139446

   Purpose

   Lentiviral vector genome to ectopically express Firefly luciferase

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag Firefly luciferase
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLSSmKate2-N1

   Plasmid

   #31867

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   LSS-mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167D/T183S/M196V

   Promoter

   CMV

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2862

   Plasmid

   #143645

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SREBF1 (SREBF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti_MCP-LSD1_Hygro

   Plasmid

   #138457

   Purpose

   lenti vector encoding the MS2-LSD1 with a 2A Hygro resistance marker

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   LSD1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-nEF Coff/Fon DREADD Gq-mCherry

    Plasmid

    #183534

    Purpose

    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Flp but not Cre

    Depositor

    Karl Deisseroth

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    nEF

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3 1 kb prom and exon1 fragment CD274

    Plasmid

    #107006

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Syn-CD4-sTb(C)-M13-GFP

    Plasmid

    #219784

    Purpose

    sTb(C) half of CaST for neuron expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CD4-sTb(C)-M13-GFP
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. lis-1::L4440

    Plasmid

    #11336

    Depositor

    Guy Caldwell

    Insert

    lis-1 (lis-1 Nematode)
    

    Use

    RNAi

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pDONR221_SLC39A6

    Plasmid

    #132153

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC39A6 (SLC39A6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

    Plasmid

    #112021

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta/alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. MXS_CMV::ZeoR-bGHpA

    Plasmid

    #62441

    Purpose

    MXS_chaining vector with CMV::ZeoR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Zeocin with CMV Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. CoV2-Spike-D614G

    Plasmid

    #177960

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-SLC25A12_STOP

    Plasmid

    #161104

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A12 (SLC25A12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCMVTNT-T7-KPNA3

    Plasmid

    #26679

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 . 11():63.

    Insert

    Karyopherin Alpha 3 (KPNA3 Human)
    

    Use

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCR-XL-rAkap9

    Plasmid

    #196867

    Purpose

    Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akap9 (Akap9 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac promoter and T7 promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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