We narrowed to 19,677 results for: Cre

1. pAAV-hSyn-Cepheid1s-ST-WPRE

   Plasmid

   #214968

   Purpose

   Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for better photostability

   Depositor

   Peng Zou

   Article

   Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

   Insert

   Cepheid1s-ST
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

   Plasmid

   #89873

   Purpose

   expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   bArrestin2-TevC-P2A-TdTomato
   

   Use

   AAV

   Tags

   TdTomato

   Expression

   Mammalian

   Mutation

   R173H in TdTomato (Please see depositor comments …

   Promoter

   Human synapsin

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJC6-GLS-3xFLAG

   Plasmid

   #163450

   Purpose

   lentiviral overexpression vector for human GLS

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   GLS (GLS Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   See: comments

   Promoter

   Ubc

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-sgRNA-lib 2.0

   Plasmid

   #89638

   Purpose

   sgRNA expression in mammalian cells after Gateway cloning

   Depositor

   Wensheng Wei

   Article

   Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Plxna2-Fc-His

   Plasmid

   #72123

   Purpose

   Expresses the extracellular region of the PlexinA2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Plxna2 (Plxna2 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AMA1-bio

   Plasmid

   #47741

   Purpose

   Expresses enzymatically monobiotinylated full-length AMA1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised AMA1
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS_L_MARV

   Plasmid

   #135473

   Purpose

   Mammalian expression vector encoding Marburg virus L gene under control of the AG promoter

   Depositor

   Elke Mühlberger

   Article

   Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

   Insert

   L gene
   

   Expression

   Mammalian

   Mutation

   silent mutation (C to T) at position 7861; insert…

   Promoter

   AG

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330 PLIN2

   Plasmid

   #202196

   Purpose

   pX330 backbone expressing sgRNA to edit the C-terminus of human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. scaffold-linker-U6

   Plasmid

   #89639

   Purpose

   construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

   Depositor

   Wensheng Wei

   Article

   Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-Puro-sgNUAK1

    Plasmid

    #138692

    Purpose

    Expresses a human NUAK1-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgNUAK1 human (NUAK1 Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid

    #138143

    Purpose

    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    NLS

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRP.ExTri-CMV-5'UTR-ApoB48-Linker-eGFP

    Plasmid

    #138334

    Purpose

    Expresses APOB-Linker-eGFP

    Depositor

    Mahmood Hussain

    Article

    Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119

    Insert

    5'UTR-ApoB48-Linker-eGFP (APOB Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

    Plasmid

    #211471

    Purpose

    lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    Cas9_T2A_mNeonGreen_P2A_BlasticidinR
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    hU6, EF-1a

    Available Since

    March 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTT3 Unc5B-FLAG

    Plasmid

    #72195

    Purpose

    Expresses full-length Unc5b with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5b (Unc5b Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSI2-ADA

    Plasmid

    #166967

    Purpose

    Express the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADAR

    Depositor

    Michael Kharas

    Article

    Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

    Insert

    MSI2-ADA
    

    Use

    Retroviral

    Tags

    MSI2-ADA

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 NPY pHCitron

    Plasmid

    #235175

    Purpose

    Plasmid expressing NPY tag with pH sensor, pHCitron (pH-Lemon with mCitrine), in mammalian cells

    Depositor

    Kate White

    Article

    Lin et al ACS Sens. 2024 Nov 22;9(11):6032-6039. d

    Insert

    NPY-pHCitron (NPY Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSI2-DCD

    Plasmid

    #166968

    Purpose

    Express the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADAR

    Depositor

    Michael Kharas

    Article

    Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

    Insert

    MSI2-DCD
    

    Use

    Retroviral

    Tags

    MSI2-DCD

    Expression

    Mammalian

    Mutation

    N495S

    Promoter

    MSCV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

    Plasmid

    #194279

    Purpose

    Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

    Inserts

    humanized VP64 dSaCas9 VP64 T2A Thy1.1
    gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hU6 and hUbC

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR

    Plasmid

    #192939

    Purpose

    FlpO-based digitizer circuit driven by AP1 promoter

    Depositor

    Wilson Wong

    Article

    Wong et al Nat Commun. 2021 Oct 22;12(1):6150. doi:

    Inserts

    AP1(2)/minTK promoter
    FlpO-ABI
    PYL-FlpO
    CAG promoter
    frt-STOP-frt
    GFP
    luciferase
    pPGK
    Zeocin resistance
    BFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode

    Plasmid

    #170248

    Purpose

    Barcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Mutation

    C124S

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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