We narrowed to 19,677 results for: Cre

1. Tol2-lyzC-mcherry-2a-CDK2

   Plasmid

   #121131

   Purpose

   neutrophil specific expression

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   dre-WT CDK2 (cdk2 Zebrafish)
   

   Tags

   mcherry-2a

   Expression

   Bacterial

   Promoter

   neutrophil specific lyzC

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

   Plasmid

   #172984

   Purpose

   CRISPRi for TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   sgTAOK2i (TAOK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dnase1L3 R206C pIRES-DsRed

   Plasmid

   #98847

   Purpose

   Expresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRed

   Depositor

   Peter Keyel

   Article

   Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

   Insert

   Dnase1L3 (DNASE1L3 Human)
   

   Tags

   IRES DsRed

   Expression

   Mammalian

   Mutation

   R206C which greatly reduces nuclease activity

   Promoter

   CMV

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

   Plasmid

   #139086

   Purpose

   Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_UdgX-APOBEC1-UdgX-HF-nCas9

   Plasmid

   #163559

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-APOBEC1-UdgX-HF-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 2PH-PLCd-APEX2-P2A-mKate2

   Plasmid

   #71284

   Purpose

   Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   2PH-PLCd-APEX2-P2A-mKate2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-delta133HtrA-EGFP

   Plasmid

   #14124

   Depositor

   L. Miguel Martins

   Article

   Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

   Insert

   HtrA2 (HTRA2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of the first 133 amino-acids

   Available Since

   March 7, 2007

   Availability

   Academic Institutions and Nonprofits only

8. EF1a_KLF16_P2A_Hygro_Barcode

   Plasmid

   #120502

   Purpose

   Barcoded lentiviral vector to express KLF16 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF16 (KLF16 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CHRM1-DuET

   Plasmid

   #213207

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CHRM1 (CHRM1 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MRAP_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176937

    Purpose

    Mammalian expression vector encoding MRAP and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    MRAP (MRAP Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sema7a-Fc-His

    Plasmid

    #72175

    Purpose

    Expresses the extracellular region of the Sema7A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema7a (Sema7a Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-Puro-sgSNRK

    Plasmid

    #138694

    Purpose

    Expresses a human SNRK-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSNRK human (SNRK Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. HT028_FCMV_CheRiff_EGFP

    Plasmid

    #178821

    Purpose

    Expresses a blue-light activated cationic channelrhodopsin CheRiff carrying an EGFP tag

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    CheRIff_EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pD2529-CAG-b3

    Plasmid

    #189797

    Purpose

    Expression of full length human integrin beta 3

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    integrin b3 (ITGB3 Human)
    

    Tags

    -P2A-mCherry

    Expression

    Mammalian

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5FRT/TO-VCPIP1-Strep-HA

    Plasmid

    #113482

    Purpose

    Expression of human VCPIP1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    VCPIP1 (VCPIP1 Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-TAF5-FL

    Plasmid

    #209047

    Purpose

    Gateway-compatible entry vector, with insert of the full length TAF5 gene

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 full length
    

    Use

    Gateway entry vector

    Expression

    Mammalian

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. aMHC-Puro Rex-Blast

    Plasmid

    #21231

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    alpha myosin heavy chain promoter
    

    Use

    Lentiviral

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_UdgX-Anc689-UdgX-nCas9-RBMX

    Plasmid

    #163552

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-Anc689-UdgX-nCas9-RBMX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKD279.8

    Plasmid

    #173171

    Purpose

    Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

    Depositor

    Jeffrey Tabor

    Article

    Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

    Inserts

    SO_4388(REC)-PsdR(DBD) 137
    sfGFP
    

    Expression

    Bacterial

    Promoter

    J23108 and PpsdA110

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLC-HA active Nedd8-P2A-Hygro

    Plasmid

    #124307

    Purpose

    Lentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    Nedd8 (NEDD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Five C-terminal amino acids missing compared to w…

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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