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pDONR223 SARS-CoV-1 NSP6_stop

Plasmid

#168548

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP6 (ORF1ab )

Use

Other

Available Since

Jan. 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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TFORF1128

Plasmid

#141798

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

THAP3 (THAP3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Kv1.4 K+ channel (extracellular) [L71/22]

Plasmid

#190291

Purpose

Mammalian Expression Plasmid of anti-Kv1.4 K+ channel (extracellular) (Rat). Derived from hybridoma L71/22.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv1.4 K+ channel (extracellular) (Rattus norvegicus) recombinant Mouse monoclonal antibody (Kcna4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis

Plasmid

#175801

Purpose

Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Tags

AviTag, Hexahistidine tag, and eGFP

Expression

Bacterial

Mutation

E184Q W330Y

Promoter

T7

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRCA892 - TFORF Empty Vector Puro-T2A-GFP (pRCA886 backbone)

Plasmid

#238180

Purpose

Lentiviral empty vector

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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SNAP-OMP25

Plasmid

#141149

Purpose

Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Budding Yeast, Rat)

Tags

SNAP tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-TBK1

Plasmid

#23851

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TBK1 (TBK1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-TIR1-HA

Plasmid

#172610

Purpose

Lentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

TIR1

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

Tet/ON Minimal CMV promoter

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMRXIP TMEM41B-3xFLAG

Plasmid

#117415

Purpose

Expresses TMEM41B tagged with 3xFLAG in mammalian cells

Depositor

Noboru Mizushima

Article

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

TMEM41B (TMEM41B Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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NSUN2

Plasmid

#188059

Purpose

Bacterial expression plasmid for human NSUN2; for activity testing

Depositor

Tanja Schirmeister

Article

Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

Insert

NSUN2 (NSUN2 Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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eNme2C-CBE6b

Plasmid

#215830

Purpose

Express CBE6b (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b-eNme2C-2xUGI

Expression

Mammalian

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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TEVSH

Plasmid

#125194

Purpose

His-tagged TEV protease with solubility increasing mutations

Depositor

Helena Berglund

Article

van den Berg et al J Biotechnol. 2006 Feb 10;121(3):291-8.

Insert

TEV_SH

Tags

V5 His-tag

Expression

Bacterial

Promoter

T7lac

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pHCD1B

Plasmid

#102953

Purpose

mammalian expression of CD1B

Depositor

Gennaro De Libero, Lucia Mori

Article

Manolova et al Eur J Immunol. 2003 Jan;33(1):29-37.

Insert

CD1B cDNA (CD1B Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2-mDux

Plasmid

#138320

Purpose

Dox-inducible codon-optimized mouse Dux

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

Dux (Dux Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-neo-Myc-Rabex-5

Plasmid

#196937

Purpose

Expresses Myc-tagged Rabex-5 in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

Insert

Rabex-5 (RABGEF1 Bovine)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-DsbC

Plasmid

#202485

Purpose

Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Escherichia coli, Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA BfuAI stuffer

Plasmid

#50920

Purpose

U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pSB-XPRESSO-hSpCas9-EGFP

Plasmid

#237298

Purpose

Sleeping Beauty XPRESSO vector for Cas9-EGFP expression

Depositor

Lior Gepstein

Article

Wexler et al Stem Cell Reports. 2025 Aug 19:102603. d

Insert

spCas9 (cas9 Stretococcus pyogenes)

Use

CRISPR

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pBT277

Plasmid

#122608

Purpose

express evoCDA1-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoCDA1-BE4max

Expression

Mammalian

Mutation

F23S A123V I195F

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-BMPR2

Plasmid

#23669

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BMPR2 (BMPR2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only