163,421 results

1. pITGA4-GFP

   Plasmid

   #81178

   Purpose

   Mammalian expression of human full length ITGA4-EGFP

   Depositor

   Chinten James Lim

   Insert

   ITGA4 (ITGA4 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains KLGFFKR motif; A1002L (please see deposi…

   Promoter

   CMV

   Available since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNH-TrxT

   Plasmid

   #26106

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Tags

   6xHis, TEV cleavage site, and TrxA

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmiRGLO Dual-Luciferase-MYB-3'UTR

   Plasmid

   #182250

   Purpose

   Human MYB 3` UTR region containing 3x binding sites for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of MYB gene
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mTagBFP2-Rab5a-7

   Plasmid

   #55322

   Purpose

   Localization: Ras GTPase, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   Rab5a
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. CMPE-ePPEplus

   Plasmid

   #205242

   Purpose

   For multiplex plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Promoter

   Available since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDF-GFPplus

   Plasmid

   #172718

   Purpose

   Expression of a fast folding GFP variant GFP plus

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   GFP plus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

   Plasmid

   #187588

   Purpose

   Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   NLS
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pColE1-ISceI

   Plasmid

   #179578

   Purpose

   Expresses I-SceI from an arabinose promoter

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   I-SceI (I-SceI Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLH-sgRNA1-2XMS2

   Plasmid

   #75389

   Purpose

   sgRNA1-2XMS2

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-2XMS2
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCoofy57

    Plasmid

    #122009

    Purpose

    Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His8 and Multi1: depending on the LP2 primer, eit…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HA-MKRN1

    Plasmid

    #78745

    Purpose

    To overexpress MKRN1 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Dat - EGFP - Tol2

    Plasmid

    #85418

    Purpose

    for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene

    Depositor

    Marc Ekker

    Article

    Xi et al Dev Dyn. 2011 Nov;240(11):2539-47. doi:

    Insert

    dopamine transporter (slc6a3 Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    dat

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

13. MYH11-NanoLuc-2A-tdTomato

    Plasmid

    #132569

    Purpose

    Donor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele

    Depositor

    James Thomson

    Article

    Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

    Insert

    Human MYH11 knockin homology arms (MYH11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ10873

    Plasmid

    #183961

    Purpose

    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG and U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-mE1

    Plasmid

    #32534

    Depositor

    Jorge Eduardo Azevedo

    Article

    Carvalho et al Mol Biotechnol. 2011 Oct 20.

    Insert

    E1, ubiquitin-activating enzyme (Uba1 Mouse)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCB’

    Plasmid

    #162703

    Purpose

    pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    pHnCB10 derived carboxysome operon, prk
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    5513T>G (tetR E37A); 7758G>T (second tet op…

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmEGFP_a_tubulin_C1

    Plasmid

    #21039

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Use

    Tags

    pmEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid

    #104567

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    

    Use

    CRISPR

    Tags

    NLS-3xHA-VPR

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. LRTX_ctrl

    Plasmid

    #214073

    Purpose

    Luminescent reporter system, NMD negative control. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Remnant of linker sequence present at the end of …

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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