179,813 results
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Plasmid#1179DepositorAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2
Plasmid#221225PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAID-PcrA M6-UGI-T2A-mTagBFP2
ExpressionMammalianAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-FLEX-CybSEP2-WPRE
Plasmid#207661PurposeMonitoring neuropeptide releaseDepositorInsertCytochrome b-561 (Cyb561 Mouse)
UseAAVMutationHistidine 86 and 159 to alanines, inserted 2 X SE…PromoterhSynapsinAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-bioGEF-UBnc-PURO
Plasmid#208044PurposeEnables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycinDepositorInsertUBnc (UBC Human)
UseLentiviralTagsLow-affinity AvitagMutationL73P Mutation near C-terminus suppresses cleavage…PromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHCMV_COCVmut_G
Plasmid#207262PurposeMammalian expression of a mutant Cocal virus G proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCOCVmut_G
ExpressionMammalianMutationK47A/Y209A/R354A; mammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-NE2h
Plasmid#208687PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neuronsDepositorHas serviceAAV5InsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseAAVPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8001 - pCMV-R8.91 (D64V)
Plasmid#202687PurposeR8.91 plasmid with mutated integrase (D64V)DepositorInsertgag pol
UseLentiviralExpressionMammalianMutationD64VAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER21-puro_Gateway-3xFLAG
Plasmid#172981PurposeGateway Destination vector for doxycycline-inducible expression of ORFDepositorTypeEmpty backboneUseLentiviralTags3xFLAGExpressionMammalianAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-KP1
Plasmid#206504PurposeAAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoterDepositorInsertKP1 capsid
UseAAVPromoterp5Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBUE411-GGB
Plasmid#198233PurposeWheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411DepositorPublicationInsertsGRF4-GIF1 chimera cassette
pPLTP::BBM cassette
UseCRISPR; Plant binary vectorExpressionPlantPromoterUbiquitin and maize PLTPAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-11b_PcrA_M6_H93A
Plasmid#194156PurposeEngineered PcrA_M6 helicase to support isothermal amplification method SHARPDepositorInsertEngineered helicase PcrA_M6
Tags6x His tag and FLAG tagExpressionBacterialMutationH93A, C96A, N187C, C247A, L384V, L409CAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-bARGSer-AxeTxe
Plasmid#192473PurposeSecond-generation bacterial acoustic reporter gene derived from SerratiaDepositorInsertsbARGSer
AxeTxe
ExpressionBacterialMutationthe gene Ser39006_001280 was deleted from the clu…PromoterNative promoter from Enterococcus faecium and pBADAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
cytoplasmic cpVenus control
Plasmid#186788PurposeIdentical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligaseDepositorInsertcytoplasmic cpVenus control
UseLentiviralExpressionMammalianAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ-Cas9-Act3.0
Plasmid#178954PurposeIt consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.DepositorInsertzCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRExpressionPlantAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
AKT-translocation-mStrawberry reporter
Plasmid#158685PurposeThis plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianPromoterEF1aAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0750 pCAG-NLS-BceINT
Plasmid#179112PurposeBceINT expression vectorDepositorInsertpCAG-NLS-BceINT
UseCRISPRAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
Plasmid#171617PurposeAAV Soma-targeted Cal-Light with KA2DepositorInsertTM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
UseAAV and Synthetic BiologyTagsMycExpressionMammalianPromoterCMVAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
VPS13D^EGFP
Plasmid#174109PurposeThe insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCodon optimized VPS13D
TagsEGFPExpressionMammalianMutationWith an internal EGFP tag in aminoacid position 1…PromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-cytoEKAR4
Plasmid#174438PurposeCytosol-targeted EKAR4.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcytoEKAR4
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHyo245
Plasmid#173147PurposeExpresses pheS_A294G by dual T7 promoter in e.coliDepositorInsertpheS_A294G
ExpressionBacterialAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only