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Signaling diversity enabled by Rap1-regulated plasma membrane ERK with distinct temporal dynamics.
Keyes J, Ganesan A, Molinar-Inglis O, Hamidzadeh A, Zhang J, Ling M, Trejo J, Levchenko A, Zhang J
Elife. 2020 May 26;9. pii: 57410. doi: 10.7554/eLife.57410. PubMed Article

Plasmids from Article

ID Plasmid Purpose
174437pcDNA3-EKAR4Improved cyan/yellow FRET-based ERK kinase activity reporter.
174438pcDNA3-cytoEKAR4Cytosol-targeted EKAR4.
174439pcDNA3-nuclear-EKAR4Nuclear-targeted EKAR4.
174440pcDNA3-pmEKAR4Plasma membrane-targeted EKAR4.
174441pcDNA3-pmEMBerPlasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.

Antibodies from Article