161,273 results

1. HA-AR-LBD

   Plasmid

   #171239

   Purpose

   Mammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   AR truncate

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SUV[SET]-dCas9

   Plasmid

   #100088

   Purpose

   Catalytic domain [SET] of human SUVAR39H1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   SUVAR39H1 (SUV39H1 Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-SUV[SET]-dCas9-NLS

   Expression

   Mammalian

   Mutation

   deleted aa 1-76

   Promoter

   CMV

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lifeact-3xmNG::HIS3

   Plasmid

   #227596

   Purpose

   Integration vector for expressing Lifeact-3xmNeonGreen

   Depositor

   Bruce Goode

   Article

   Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

   Insert

   Lifeact-3xmNeonGreen::HIS3
   

   Use

   Tags

   3xmNeonGreen

   Expression

   Yeast

   Mutation

   Truncated ABP140 (aa 1-17 only)

   Promoter

   Abp140

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. COVID-SARS2 NSP13

   Plasmid

   #159614

   Purpose

   Bacterial expression plasmid for COVID-SARS2 NSP13 helicase

   Depositor

   Opher Gileadi

   Article

   Newman et al Nat Commun. 2021 Aug 11;12(1):4848. doi:

   Insert

   Covid-SARS2 Nsp13
   

   Use

   Tags

   His6-Zb-TEV

   Expression

   Bacterial

   Mutation

   Codon-optimized for E. coli expression

   Promoter

   T7 - LacO

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF3008

   Plasmid

   #144484

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CDX2 (CDX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-puro-hMGT

   Plasmid

   #172393

   Purpose

   Retroviral expression vector for human direct cardiac reprogramming

   Depositor

   Li Qian

   Article

   Zhou et al Cell Stem Cell. 2019 Jul 3;25(1):149-164

   Insert

   Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMGF182

   Plasmid

   #97006

   Purpose

   Expresses GFP-CHMP7 in mammalian cells under a crippled promoter

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   CHMP7 (CHMP7 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV delta 5

   Available since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-Bsd-CMV-MDM4

   Plasmid

   #202669

   Purpose

   MDM4 overexpression vector with blasticidin resistance

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 regulator of p53 (MDM4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9

   Plasmid

   #46569

   Purpose

   Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   dcas9
   CRISPR array
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7C-HSP90AB1

    Plasmid

    #177659

    Purpose

    Recombinant protein synthesis of human HSP90AB1

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSP90AB1 (HSP90AB1 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. modified pcAT7-Glo1

    Plasmid

    #160996

    Purpose

    Splicing reporter backbone

    Depositor

    Brenton Graveley

    Article

    Adamson et al Genome Biol. 2018 Jun 1;19(1):71. doi: 1

    Insert

    hemoglobin subunit beta (HBB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Repeated intron 1 with 40 nucelotide deletion

    Promoter

    CAG

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV-U6+27-tCorn

    Plasmid

    #106233

    Purpose

    Express tRNA-Corn as a reporter of U6 promoter activity in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

    Insert

    tRNA-Corn
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-SrtAdelta59

    Plasmid

    #51138

    Purpose

    expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.aureus SrtA delta 59
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pB-Neo-mtkeima

    Plasmid

    #210338

    Purpose

    Overexpression of mtkeima

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    mtkeima
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP_TRPM8

    Plasmid

    #64879

    Purpose

    Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

    Depositor

    Sebastián Brauchi

    Article

    Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

    Insert

    Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLSSmOrange-N1

    Plasmid

    #37130

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

    Insert

    LSSmOrange
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

    Promoter

    CMV

    Available since

    June 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-tetO-myc T58A

    Plasmid

    #19763

    Depositor

    Konrad Hochedlinger

    Article

    Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

    Insert

    c-myc (MYC Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2-39

    Plasmid

    #118086

    Purpose

    ZB-linker-split C-terminal T7 RNAP expression plasmid

    Depositor

    Bryan Dickinson

    Article

    Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

    Insert

    ZB-linker-split C-terminal T7 RNAP expression plasmid
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFR_CrPV_xb

    Plasmid

    #11509

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pl-Synapsin-YFP-NaVII-III

    Plasmid

    #91246

    Purpose

    Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)

    Depositor

    Matthew Grubb

    Article

    Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268.

    Insert

    Nav1.2 (Scn2a Rat)
    

    Use

    Lentiviral

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    The insert codes only for the intracellular loop …

    Promoter

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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