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cfSGFP2-col4a1
(Plasmid #160317)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160317 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 160317-D50 50 μg of DNA in Tris buffer $495
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DNA 160317-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    EGFP-N1
  • Backbone manufacturer
    Clonetech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    COL4A1
  • Species
    H. sapiens (human)
  • Entrez Gene
    COL4A1 (a.k.a. BSVD, BSVD1, COL4A1s, PADMAL, RATOR)
  • Tag / Fusion Protein
    • cfSGFP2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains a T824P mutation in the insert (lies within the Mature Domain). This variant is not known to affect plasmid function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cfSGFP2-col4a1 was a gift from Ikuo Wada (Addgene plasmid # 160317 ; http://n2t.net/addgene:160317 ; RRID:Addgene_160317)

  • For your References section:

    Visualization of Procollagen IV Reveals ER-to-Golgi Transport by ERGIC-independent Carriers. Matsui Y, Hirata Y, Wada I, Hosokawa N. Cell Struct Funct. 2020 Jul 23;45(2):107-119. doi: 10.1247/csf.20025. Epub 2020 Jun 18. 10.1247/csf.20025 PubMed 32554938
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