167,150 results

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FLAG-CBLB

Plasmid

#192082

Purpose

Mammalian expression of human CBLB with FLAG tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

CBLB (CBLB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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NbAlfa-GFP2

Plasmid

#201485

Purpose

BRET2 detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbAlfa-GFP2

Tags

GFP2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3

Plasmid

#209727

Purpose

Expresses cytosolic medium affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95K.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-RhoA-wt

Plasmid

#12965

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA (RHOA Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pCROPseq-VEX-optimized-scaffold

Plasmid

#203321

Purpose

Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2055

Plasmid

#142178

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CDX1 (CDX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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tubP-FRT>GAL80-6-FRT>

Plasmid

#63173

Purpose

ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"Flp-Out" GAL80

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pRE107

Plasmid

#43829

Depositor

Dieter Schifferli

Article

Edwards et al Gene. 1998 Jan 30;207(2):149-57.

Type

Empty backbone

Use

Bacterial allelic exchange vector with sacb1

Expression

Bacterial

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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PTGDR-Tango

Plasmid

#66482

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

PTGDR (PTGDR Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pFUW PuroR-P2A-COX8ASig-Timer

Plasmid

#239559

Purpose

Expression and mitohcondrial localization of Timer

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

Timer

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro Xlone-eGFP

Plasmid

#136936

Purpose

donor plasmid for inducible transgene expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

all-in-one tet-on system

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3GS

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-SCGB3A2 promoter-EGFP-EF1a-TagRFP

Plasmid

#204821

Purpose

Lentiviral vector for human SCGB3A2 promoter-driven expression of EGFP

Depositor

Emma Rawlins

Article

He et al Cell. 2022 Dec 8;185(25):4841-4860.e25.

Insert

An upstream region including human SCGB3A2 promoter

Use

Lentiviral

Expression

Mammalian

Promoter

SCGB3A2 promoter

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pScRad52-SpCas9 (pXK-1646)

Plasmid

#208823

Purpose

CRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.

Depositor

Kun Xu

Article

Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

Type

Empty backbone

Use

CRISPR; Cas9

Tags

Rad52

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pTW2066

Plasmid

#236575

Purpose

ISYmu1 PDS3 gRNA2 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAGM51323

Plasmid

#153224

Purpose

Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMA7CR_2.0

Plasmid

#73950

Purpose

pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

Depositor

Alex Nielsen

Article

Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

Inserts

cas9
lambda beta
dam
recX

Expression

Bacterial

Promoter

pAra and pTet

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

Plasmid

#67986

Purpose

Cas9 activity reporter with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-Puro-2A

Plasmid

#102611

Purpose

Control lentivector expressing Puro resistance and gene of interest from CMV promoter

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA/TO/EGFPmiR21

Plasmid

#10827

Depositor

Thomas Tuschl

Article

Meister et al RNA. 2004 Mar . 10(3):544-50.

Insert

pcDNA/TO/EGFPmiR21

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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ND4.2-Left TALE-G1397-N-DddA11-mCherry

Plasmid

#179682

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR

Expression

Mammalian

Mutation

S1330I A1341V N1342S E1370K T1380I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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