Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

179,844 results

Showing: 10341 - 10360 of 179844 results

  2. pDEST14-Cys-SpyCatcher002

    Plasmid
    #102829
    Purpose
    Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents
    Depositor
    Mark Howarth
    Publication
    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi
    Insert
    Cys-SpyCatcher002
    Tags
    GSSGS, His6, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Cysteine residue in N-terminal region
    Promoter
    T7
    Available since
    Dec. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102829-plasmid-map-sequence-id-200986

    Enlarge

  5. px459 VQR

    Plasmid
    #101715
    Purpose
    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VQR
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VQR (D1135V, R1335Q and T1337R)
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101715-plasmid-map-sequence-id-196971

    Enlarge

  6. pGL4-AARE-luc2P-Hygro

    Plasmid
    #101787
    Purpose
    Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)
    Depositor
    Seiichi Oyadomari
    Publication
    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xAARE
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Promoter
    minimal TATA-box promoter with low basal activity
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101787-plasmid-map-sequence-id-194135

    Enlarge

  11. pl-Synapsin-YFP-NaVII-III

    Plasmid
    #91246
    Purpose
    Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)
    Depositor
    Matthew Grubb
    Publication
    Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268.
    Insert
    Nav1.2 (Scn2a Rat)
    Use
    Lentiviral
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    The insert codes only for the intracellular loop …
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91246-plasmid-map-sequence-id-180624

    Enlarge

  12. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Publication
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

    Enlarge

Showing: 10341 - 10360 of 179844 results