162,999 results

1. pmCherry-C1-mTrim21

   Plasmid

   #105516

   Purpose

   Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLI50

   Plasmid

   #13573

   Depositor

   Chia Lee

   Article

   Lee et al Gene. 1991 Jul 15. 103(1):101-5.

   Type

   Empty backbone

   Use

   S. aureus-e. coli shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-REPORT (PGK) NanoLuc

   Plasmid

   #208576

   Purpose

   Empty NanoLuc Lentiviral reporter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   NanoLuciferase
   d2eGFP
   Empty response element
   

   Use

   Lentiviral, Luciferase, and Synthetic Biology

   Tags

   3x SV40 NLS and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   PGK and miniP

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-ZDHHC8

   Plasmid

   #231657

   Purpose

   Expresses human ZDHHC8 protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC8 (ZDHHC8 Human)
   

   Use

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

   Plasmid

   #20943

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CaMKIIa

   Available since

   July 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3258 pCMV hUBC9 wt HA

   Plasmid

   #14438

   Depositor

   Peter Howley

   Article

   Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

   Insert

   UBC9 (UBE2I Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4-Flag-Timeless

   Plasmid

   #22887

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2005 Apr . 25(8):3109-16.

   Insert

   Timeless (TIMELESS Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted normal start codon, replaced with start c…

   Promoter

   Available since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAK_DR30_EF1a_CasRx_Puro

   Plasmid

   #134842

   Purpose

   Lentiviral all in one expression of CasRx and guideRNA

   Depositor

   Michael Yaffe

   Insert

   RfxCas13d
   

   Use

   CRISPR and Lentiviral

   Tags

   T2A Puromycin

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV MP-135 mRFP

   Plasmid

   #206927

   Purpose

   The micro promoter MP-135 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (135 bp) allows delivery of large genes in AAV.

   Depositor

   Markus Grompe

   Article

   Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

   Insert

   mRFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MP-135

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-UBC-GCAMP6f-PGK-neo

    Plasmid

    #160049

    Purpose

    expression of GCaMP6f in a piggybac plasmid

    Depositor

    Xiaojun Lian

    Insert

    GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSIN-TRE-H2BeGFP-rtTA2

    Plasmid

    #165494

    Purpose

    Doxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.

    Depositor

    Hector G. Palmer

    Article

    Puig et al J Clin Invest. 2018 Aug 31;128(9):3887-3

    Insert

    Human histone H2B (H2BC21 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    -

    Promoter

    TetO-CMV

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. HyWnt3:Luc

    Plasmid

    #192986

    Purpose

    Plasmid to test the Hydra Wnt3 promoter activity

    Depositor

    Brigitte Galliot

    Article

    Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

    Insert

    Hydra Wnt3 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-PTEN

    Plasmid

    #13039

    Depositor

    Alonzo Ross

    Article

    Liu et al J Cell Biochem. 2005 Oct 1. 96(2):221-34

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pORANGE GFP-Camk2a KI

    Plasmid

    #131484

    Purpose

    Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Camk2a, Camk2a Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACUH

    Plasmid

    #58374

    Purpose

    UAS vector to express gene of interest in Drosophila

    Depositor

    Chun Han, Yuh-Nung Jan

    Article

    Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid

    #217808

    Purpose

    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

    Plasmid

    #63166

    Purpose

    GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-HA"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV_Cas9_puro

    Plasmid

    #65655

    Purpose

    Retroviral introduction of Cas9 into mammalian cell line.

    Depositor

    Christopher Vakoc

    Article

    Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    5 ' FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    MSSV_LTR

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAK_DR30_CasRx_mCherry

    Plasmid

    #134844

    Purpose

    All in one overexpression of CasRx and guideRNA

    Depositor

    Michael Yaffe

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Il12b 400bp pro-Luc

    Plasmid

    #20020

    Depositor

    Stephen Smale

    Article

    Plevy et al Mol Cell Biol. 1997 Aug . 17(8):4572-88.

    Insert

    Il12b promoter (Il12b Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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