Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells.
Lambrus BG, Moyer TC, Holland AJ
Methods Cell Biol. 2018;144:107-135. doi: 10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5. PubMed Article
Lambrus BG, Moyer TC, Holland AJ
Methods Cell Biol. 2018;144:107-135. doi: 10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 80073 | pBabe Blast osTIR1-9Myc | Retroviral vector for stable expression of osTIR1-9Myc in mammalian cell lines |
| 80074 | pBabe Puro osTIR1-9Myc | Retroviral vector for stable expression of osTIR1-9Myc in mammalian cell lines |
| 101713 | pcDNA5/FRT miniAID-EGFP | For amplification of a miniAID-EGFP for C-terminal tagging |
| 101714 | pcDNA5/FRT EGFP-miniAID | For amplification of a EGFP-miniAID for N-terminal tagging |
| 101715 | px459 VQR | sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM) |
| 101716 | px459 VRER | sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM) |
| 101727 | p458 VQR | Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs |
| 101728 | p458 VRER | Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs |
| 101729 | px330 VRER | Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs |
| 101730 | p330 VQR | Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs |
| 101731 | px458 EQR | Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs |
| 101732 | px459 EQR | Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs |
| 101733 | px330 EQR | Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs |