165,343 results

1. pRDA_867

   Plasmid

   #231109

   Purpose

   A>G base editor

   Depositor

   John Doench, David Root

   Insert

   ABE8e-nCas9-SpG-HA
   

   Use

   CRISPR and Lentiviral

   Mutation

   D10A

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. p6599 MSCV-IP N-HAonly FOSL1

   Plasmid

   #34897

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   FOSL1 (FOSL1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pInducer20 ALPK1 Y254C

   Plasmid

   #231587

   Purpose

   ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

   Depositor

   Thomas Henry

   Article

   Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

   Insert

   ALPK1 (ALPK1 Human)
   

   Use

   Lentiviral

   Tags

   3X flag

   Expression

   Mammalian

   Mutation

   changed tyrosine 254 to cysteine

   Promoter

   doxycycline

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-Sec61b-C1

   Plasmid

   #90994

   Purpose

   Expresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

   Insert

   mCherry-Sec61b (SEC61B Synthetic, Human)
   

   Tags

   mCherry (red fluorescence)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-Tre3G-29xGGGGCC-12xMS2

   Plasmid

   #99149

   Purpose

   Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector. *Please see comments section below about the number of verified repeats.

   Depositor

   Ron Vale

   Article

   Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

   Insert

   29xGGGGCC 12xMS2 hairpins
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1 EGFP-Tau

   Plasmid

   #108868

   Purpose

   Expresses EGFP linked to human Tau

   Depositor

   Gabriele Kaminski Schierle

   Insert

   Tau (MAPT Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   human four-repeat tau lacking the N-terminal sequ…

   Promoter

   CMV

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

   Plasmid

   #182352

   Purpose

   Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. B-gTEMP/pcDNA3

   Plasmid

   #188447

   Purpose

   B-gTEMP for mammalian expression

   Depositor

   Takeharu Nagai

   Article

   Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

   Insert

   B-gTEMP
   

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1+DPP6

   Plasmid

   #117272

   Purpose

   Encodes human DPP6 variant 1

   Depositor

   Mark Cannell

   Article

   Lainez et al Biochem Pharmacol. 2018 Apr;150:120-130.

   Insert

   DPP6 (DPP6 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIBN(deltaNLS)-pmGFP

    Plasmid

    #26867

    Purpose

    Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    NLS sequences mutated

    Promoter

    CMV

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

    Plasmid

    #214875

    Purpose

    Empty lentiviral backbone for guide array cloning

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-CAG-FLPX-rc [Jaws-KGC-tdTomato-ER2]

    Plasmid

    #195488

    Purpose

    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in FLPX/reversed (Frt-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-tdTomato-ER2
    

    Use

    AAV

    Tags

    ER2, KGC, and tdTomato

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CAG

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKDsgRNA-pgaC

    Plasmid

    #154919

    Purpose

    aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    gRNA targeting pgaC
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCas9-BFP

    Plasmid

    #78545

    Purpose

    Fusion protein Cas9+BFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Fusion protein with Cas9

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_PGK_LaG16_2_synNotch_Gal4VP64

    Plasmid

    #85421

    Purpose

    Lentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    LaG16_2 GFP nanobody synNotch Gal4VP64
    

    Use

    Lentiviral

    Promoter

    PGK

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-mMaroon1

    Plasmid

    #83840

    Purpose

    Expresses mMaroon1

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. VAMP3-mCherry

    Plasmid

    #92423

    Purpose

    Expression of VAMP3 C-terminally conjugated to mCherry.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    VAMP3 (Vamp3 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICSL50009

    Plasmid

    #50309

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    C terminal HA tag (6x Human influenza hemagglutinin)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-APEX2-C Cloning Vector

    Plasmid

    #128146

    Purpose

    To express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEM

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Tags

    Flag-APEX2

    Expression

    Mammalian

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFGAMS-mEos2

    Plasmid

    #105128

    Purpose

    Expresses human FGAMS-mEos2 fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    French et al Science. 2016 Feb 12;351(6274):733-7. do

    Insert

    PFAS (PFAS Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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