Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #73206)


Item Catalog # Description Quantity Price (USD)
Plasmid 73206 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5388
  • Total vector size (bp) 6459
  • Modifications to backbone
    The NdeI site was removed from the CMV promoter. The XhoI in the vector was destroyed. Another was introduced with the insert.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    No special instructions.
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L91S and NP138
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Promoter CMV
  • Tags / Fusion Proteins
    • The FAP is fused with 2 copies of a G4S linker
    • myc 1020-1049

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI/XhoI but another XhoI introduced with the insert (destroyed during cloning)
  • 5′ sequencing primer CMVF
  • 3′ sequencing primer BGHR
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.

For more information about this plasmid, please see the associated publication (PMID: 25650487) as well as He et al. Nat Methods. 2016 Jan 25. doi: 10.1038/nmeth.3735. [Epub ahead of print] PMID: 26808669.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-kappa-myc-dL5-2xG4S-TMst was a gift from Marcel Bruchez (Addgene plasmid # 73206 ; ; RRID:Addgene_73206)
  • For your References section:

    Rapid, specific, no-wash, far-red fluorogen activation in subcellular compartments by targeted fluorogen activating proteins. Telmer CA, Verma R, Teng H, Andreko S, Law L, Bruchez MP. ACS Chem Biol. 2015 May 15;10(5):1239-46. doi: 10.1021/cb500957k. Epub 2015 Feb 16. 10.1021/cb500957k PubMed 25650487