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pGolt-mCherry / pGolt3-mCherry
(Plasmid #73297)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73297 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherryN1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4773
  • Total vector size (bp) 4896
  • Modifications to backbone
    This plasmid contains the multiple cloning site (MCS) of pmCherry-N1 vector.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    XL10-Gold Ultracompetent cells should be used for multiplication of plasmid.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ER export signal of Scap
  • Alt name
    SREBF chaperone (Scap)
  • Species
    Cricetulus griseus (Chinese hamster)
  • Insert Size (bp)
    36
  • GenBank ID
    NM_001244036 NP_001230965
  • Promoter CMV immediate early promoter
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspEI (not destroyed)
  • 3′ cloning site BspEI (destroyed during cloning)
  • 5′ sequencing primer Forward primer for pEGFPN1 or pmCherryN1
  • 3′ sequencing primer Reverse primer for pmCherryN
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    ER export signal of Scap
  • Alt name
    SREBF chaperone (Scap)
  • Species
    Cricetulus griseus (Chinese hamster)
  • Insert Size (bp)
    36
  • GenBank ID
    NM_001244036 NP_001230965
  • Promoter CMV immediate early promoter
  • Tag / Fusion Protein
    • ER export (N terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspEI (destroyed during cloning)
  • 3′ cloning site BspEI (destroyed during cloning)
  • 5′ sequencing primer Forward primer for pEGFPN1 or pmCherryN1
  • 3′ sequencing primer Reverse primer for pmCherryN
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    TMD (trans-membrane domain) of Calneuron-2
  • Alt name
    calcium binding protein 7 (Cabp7)/C-terminal region of Calneuron-2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    87
  • GenBank ID
    NM_001007730 NP_001007731
  • Entrez Gene
    Cabp7 (a.k.a. rCaBP7)
  • Promoter CMV immediate early promoter
  • Tag / Fusion Protein
    • ER export (N terminal on insert)

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspEI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer Forward primer for pEGFPN1 or pmCherryN1
  • 3′ sequencing primer Reverse primer for pmCherryN
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGolt-mCherry / pGolt3-mCherry was a gift from Michael Kreutz (Addgene plasmid # 73297 ; http://n2t.net/addgene:73297 ; RRID:Addgene_73297)
  • For your References section:

    A Dendritic Golgi Satellite between ERGIC and Retromer. Mikhaylova M, Bera S, Kobler O, Frischknecht R, Kreutz MR. Cell Rep. 2015 Dec 30. pii: S2211-1247(15)01437-0. doi: 10.1016/j.celrep.2015.12.024. 10.1016/j.celrep.2015.12.024 PubMed 26748700