162,717 results

1. GFP-CA-mDia1

   Plasmid

   #45583

   Depositor

   Klaus Hahn, Ronen Zaidel-Bar

   Article

   Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

   Insert

   Constitutively active mDia1 (CA-mDia1) (Diaph1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Insert mDia1 contains mutations V161D and N165D i…

   Promoter

   CMV

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

2. HTR1A-Tango

   Plasmid

   #66404

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   HTR1A (HTR1A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MSCV_Cas9_puro

   Plasmid

   #65655

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line.

   Depositor

   Christopher Vakoc

   Article

   Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   5 ' FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   MSSV_LTR

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAK_DR30_EF1a_CasRx_Puro

   Plasmid

   #134842

   Purpose

   Lentiviral all in one expression of CasRx and guideRNA

   Depositor

   Michael Yaffe

   Insert

   RfxCas13d
   

   Use

   CRISPR and Lentiviral

   Tags

   T2A Puromycin

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a URA3

   Plasmid

   #61924

   Purpose

   Expression of S. cerevisiae URA3 cloned into pFA6a

   Depositor

   David Tollervey, Jon Houseley

   Article

   Houseley et al Nucleic Acids Res. 2011 Nov 1;39(20):877

   Insert

   URA3
   

   Use

   Yeast genomic targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-Hhex

   Plasmid

   #131600

   Purpose

   expression of mouse Hhex in mammalian cells

   Depositor

   Kateri Moore, Filipe Pereira

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   Hhex (Hhex Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6b:sgRNA#3

   Plasmid

   #64247

   Purpose

   expresses sgRNA under U6b promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZac2.1-GfaABC1D-Rpl22-HA

   Plasmid

   #111811

   Purpose

   Express Rpl22HA in astrocytes

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Ribosomal protein L22 (Rpl22 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-entry-puro

   Plasmid

   #85745

   Purpose

   Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCoofy27

    Plasmid

    #44003

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    PPH

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMSCV PIG (Puro IRES GFP empty vector)

    Plasmid

    #21654

    Purpose

    Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-CreEGFP

    Plasmid

    #165140

    Purpose

    Expression of Cre recombinase fused with EGFP in C-terminal

    Depositor

    Daekee Lee, David Threadgill

    Insert

    Cre recombinase
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmScarlet-i_C1

    Plasmid

    #85044

    Purpose

    Mammalian expression of mScarlet-i

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    mScarlet-i
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMK287 (mAID-Hygro)

    Plasmid

    #72825

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Hygro
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Non tagged Avidin

    Plasmid

    #214835

    Purpose

    This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Non tagged Avidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mScarlet-MYO10

    Plasmid

    #145179

    Purpose

    Expresses mScarlet-MYO10 in mammalian cells

    Depositor

    Johanna Ivaska

    Article

    Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

    Insert

    mScarlet-MYO10 (MYO10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. SPHK2 gRNA (BRDN0001148465)

    Plasmid

    #77093

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSL1777 (pEffector)

    Plasmid

    #160731

    Purpose

    Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins and crRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MEK1 (S218D,S222D)-pcw107

    Plasmid

    #64604

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S218D, S222D

    Promoter

    PGK

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. P362-FBP_12

    Plasmid

    #139703

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge