170,794 results
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pHAGE-IGF1R-F1057L
Plasmid#116414PurposeLentiviral expression of IGF1R F1057LDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone
Plasmid#228364PurposemU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-PuroDepositorTypeEmpty backboneUseCRISPR and Lentiviral; Enascas12a nuclease and en…ExpressionMammalianPromotermU6Available SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH1v3 (+TEV)
Plasmid#204229PurposeBacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).InserthMDH1v3 (MDH1 Human)
Tags6X HisExpressionBacterialMutationInserted a G after ATG to maintain kozak sitePromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEC3115 (pSpy0K6-P23_mKate2~*ssrA)
Plasmid#218509Purposeintegrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombinationDepositorInsertmKate2
TagsssrA degradation tagExpressionBacterialMutationDeletion of Plac_mrfp cassette, introduction of a…PromoterP23Available SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pLKO.1-TetON-Puro-HIF1a 3809
Plasmid#118705PurposeLentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)DepositorInsertshRNA against human HIF1a
UseLentiviralAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
polyQ74-GFP
Plasmid#231893PurposeForms cytosolic HTT partial exon 1 Q74 aggregatesDepositorInsertHTT partial exon 1 Q74 (HTT Human)
TagsEGFPExpressionMammalianMutationCAG repeat expansionAvailable SinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.8h.8TM.28.3z
Plasmid#200670PurposeModular CD8hinge-CD8transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianPromoterEF1a-shortAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-GST
Plasmid#42049DepositorInsertGST
TagsHisExpressionBacterialAvailable SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1-YAP
Plasmid#166457PurposeExpresses fusion of mEGFP and YAPDepositorInsertmEGFP, YAP (YAP1 Human)
ExpressionMammalianAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hMLN
Plasmid#27079PurposeNon-integrating (episomal) expression of human C-MYC, LIN28 and NANOGDepositorInsertC-MYC, LIN28, NANOG (MYC Human)
ExpressionMammalianAvailable SinceMay 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
Plasmid#221359PurposeMammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.DepositorInsertAnti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
UseAffinity Reagent/ AntibodyExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
SFFV-IKZF3-CTERM-GFP
Plasmid#185769PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, IKZF3 minidegronMutationnonePromoterSFFVAvailable SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LuxI
Plasmid#193626PurposeConstitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.DepositorInsertLuxI
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP
Plasmid#102797PurposeRetrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroRDepositorInsertssgRNA
EGFP
UseCRISPR and RetroviralExpressionMammalianMutationH231LAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-TRIM28
Plasmid#65397PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iCreV
Plasmid#140135PurposeCan be used to generate AAV virus that will express light-inducible site-specific iCreV recombinaseDepositorHas ServiceAAV PHP.eB and AAV1InsertiCreV
UseAAV and Cre/LoxExpressionMammalianPromoterEF1alphaAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEND-227_Cacnes_mCerulean
Plasmid#225614PurposeReplicative vector for blue fluorescent reporter protein mCerulean strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCerulean.DepositorInsertmCerulean
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRIM33-GFP
Plasmid#65399PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only
