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pLVX-IRES-tdTomato-FlagAkt1
(Plasmid #64831)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64831 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLVX-IRES-tdTomato
  • Backbone size w/o insert (bp) 8892
  • Total vector size (bp) 10404
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Akt1
  • Alt name
    PKBalpha
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1512
  • GenBank ID
    NM_009652
  • Entrez Gene
    Akt1 (a.k.a. Ak, Akt, LTR-akt, PK, PKB, PKB/A, PKB/Akt, PKBalpha, Rac)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFlag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GACCTCCATAGAAGACACCGACT
  • 3′ sequencing primer GCCTTATTCCAAGCGGCTTCG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-IRES-tdTomato-FlagAkt1 was a gift from Eva Gonzalez (Addgene plasmid # 64831 ; http://n2t.net/addgene:64831 ; RRID:Addgene_64831)
  • For your References section:

    Development of a new model system to dissect isoform specific Akt signaling in adipocytes. Kajno E, McGraw TE, Gonzalez E. Biochem J. 2015 Apr 9. 10.1042/BJ20150191 PubMed 25856301