Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #60488)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60488 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
    Didier Trono
  • Total vector size (bp) 8895
  • Modifications to backbone
    dRT-pMDLg/pRRE was generated by megaprimer-PCR on previously described pMDLg/pRRE using primer u44 (fw: ACAATGAGACACCA), u45 (rev: GATATGTCCATTGGCCTTGCCC) and u46 (RT-mtagenesis-rev: TACATACAACACCACCATGTAT) to mutate the active centre of the reverse transcriptase by replacing YMDD by YMVV.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    any (like TOP10, XL10-Gold or Stbl)
  • Copy number
    High Copy


  • Gene/Insert name
    HIV-1 gag, HIV-1 pol with inactive reverse transcriptase
  • Alt name
  • Mutation
    changed Asp at position 249 + 250 to Val

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The initial backbone pMDLg/pRRE was created by Didier Trono (Addgene Plasmid #12251)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dRT-pMDLg/pRRE was a gift from Boris Fehse (Addgene plasmid # 60488 ; ; RRID:Addgene_60488)
  • For your References section:

    Novel lentiviral vectors with mutated reverse transcriptase for mRNA delivery of TALE nucleases. Mock U, Riecken K, Berdien B, Qasim W, Chan E, Cathomen T, Fehse B. Sci Rep. 2014 Sep 18;4:6409. doi: 10.1038/srep06409. 10.1038/srep06409 PubMed 25230987