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3xERRE/ERE-luciferase

Plasmid

#37852

Depositor

Rebecca Riggins

Article

Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

Insert

3 copies of ERRE/ERE upstream of luciferase

Use

Luciferase

Promoter

SV40

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CXCL12-sfGFP

Plasmid

#98961

Purpose

Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCL12 (CXCL12 Human)

Tags

Superfolder GFP

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLv-HSA-uDys/eGFP

Plasmid

#26810

Depositor

Jeffrey Chamberlain

Article

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

dystrophin (DMD Human)

Use

Lentiviral; Lentiviral plasmid backbone from l. n…

Tags

eGFP

Mutation

deleted R4-R23 deleted exons 71-78

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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RFP Dynamin2 Wild Type

Plasmid

#128152

Purpose

Expression of Dynamin 2

Depositor

Jennifer Lippincott-Schwartz

Article

Chua et al Proc Natl Acad Sci U S A. 2009 Dec 8;106

Insert

Dynamin 2 (Dnm2 Rat)

Expression

Mammalian

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-TORCAR

Plasmid

#64929

Purpose

Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

LAMP1-TORCAR (LAMP1 Human, Synthetic)

Tags

Cerulean, Full-length LAMP1, and YPet

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

Plasmid

#161926

Purpose

Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Insert

rTetR(SE-G72P)

Use

Lentiviral and Synthetic Biology

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTreTight-Htt94Q-CFP

Plasmid

#23966

Depositor

Nico Dantuma

Article

Maynard et al Proc Natl Acad Sci U S A. 2009 Aug 18. 1

Insert

N-terminal fragment Huntingtin Q94 (HTT Human)

Tags

CFP

Expression

Mammalian

Mutation

94 polyQ repeat

Available Since

April 15, 2010

Availability

Academic Institutions and Nonprofits only

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EF05J

Plasmid

#42941

Depositor

Eline Luning Prak

Article

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

Negative control Functionally Dead Human L1 element (L1RE1 Human)

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

ORF1 R261A, R262A

Available Since

April 25, 2013

Availability

Academic Institutions and Nonprofits only
pRSET-MagLOV

Plasmid

#219957

Purpose

Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

Depositor

Andrew York

Article

Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

Insert

MagLOV

Tags

6xHis

Expression

Bacterial

Mutation

AsLOV2 C450P L496V Q513K G528K D540M

Promoter

T7

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEDA5_GFPmut3_Y66H

Plasmid

#80085

Purpose

Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

Depositor

Timothy Whitehead

Article

Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

Insert

GFPmut3_Y66H

Tags

6x-His tag

Expression

Bacterial

Mutation

changed Tyrosine 66 to Histidine

Promoter

proB

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDD120

Plasmid

#119879

Purpose

Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pConstitutive_Che9c60 and Che9c61

Expression

Bacterial

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Luc-pSUPER-Retro-GFP/Neo

Plasmid

#22133

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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RN3P-KDM5B-FLAG

Plasmid

#86398

Purpose

FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

Depositor

Wei Xie

Article

Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

Insert

KDM5B (Kdm5b Mouse)

Tags

3x-FLAG

Expression

Mammalian

Promoter

Lac

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX puro TRIM21-GFP

Plasmid

#116941

Purpose

Expression of TRIM21-GFP fusion protein

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

TRIM21 - GFP (TRIM21 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGFAP-TetOn3G

Plasmid

#133728

Purpose

Plasmid contains the TetOn3G-transactivator sequence under control of a GFAP promoter.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

TetOn3G

Use

AAV

Expression

Mammalian

Promoter

pGFAP

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-AncBE4max

Plasmid

#174164

Purpose

This is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.

Depositor

Wensheng Wei

Article

Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103

Insert

AncBE4max

Use

Lentiviral

Tags

P2A-mCherry

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAIP hIL4 co

Plasmid

#74169

Purpose

Lentiviral vector for expression of human codon optimized IL4 with puromycin resistance.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

IL4 (IL4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMIIS793

Plasmid

#238206

Purpose

Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Tags

TwinStrepII-bdSUMO

Expression

Bacterial

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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T7-VEE-OKS-iG

Plasmid

#58974

Purpose

RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Inserts

Oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 Human)
GLIS (GLIS1 Human)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC20A1

Plasmid

#132010

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC20A1 (SLC20A1 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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