166,778 results

1. pICSL01009::AtU6p

   Plasmid

   #46968

   Purpose

   Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSec61β-mScarlet3-H (aka mYongHong)

   Plasmid

   #239414

   Purpose

   Expresses Sec61b-mScarlet3-H in mammalian cells

   Depositor

   Zhifei Fu

   Article

   Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

   Insert

   Sec61β (SEC61B Human)
   

   Tags

   mScarlet3-H (aka mYongHong)

   Expression

   Mammalian

   Mutation

   M163H in mScarlet3

   Promoter

   CMV

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BM00654

   Plasmid

   #155339

   Purpose

   Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. QPM-sgR (pTaU3)

   Plasmid

   #140448

   Purpose

   For sgRNA expression in monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

   Insert

   TaU3-sgRNA
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   TaU3

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FLX1.8NFATGFPpd2HS4

   Plasmid

   #169096

   Purpose

   Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation

   Depositor

   Geoffrey Owens

   Inserts

   NFAT enhancer (from IL-4 promoter)
   Destabilized green fluorescent protein
   

   Use

   Lentiviral

   Promoter

   NFAT enhancer + synthetic TATA box

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1(+)/AUG-DAP5-3XFLAG

   Plasmid

   #127331

   Purpose

   Express 3xFLAG tagged DAP5 protein from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   DAP5 (EIF4G2 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ClpP-his

   Plasmid

   #22144

   Depositor

   Robert Sauer

   Article

   Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

   Insert

   ClpP-His6
   

   Tags

   his

   Expression

   Bacterial

   Mutation

   C-terminal His tag

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLacO-ISce1

   Plasmid

   #58505

   Purpose

   provides 256 Lac operator repeats adjacent to the ISce1 restriction site

   Depositor

   Tom Misteli

   Article

   Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

   Insert

   Lac repeats adjacent to 6 copies of the ISce1 restriction site
   

   Expression

   Bacterial

   Promoter

   no promoter

   Available Since

   Oct. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDH_blast_MCS_Nard_GFP_LAMIN

   Plasmid

   #167340

   Purpose

   Expresses GFP fused to lamin A (LA) in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   Lamin A (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMIA10.7

    Plasmid

    #214451

    Purpose

    BxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Inserts

    Tet-On 3G
    PuroR
    TRE3G CopGFP
    

    Use

    Bxbi donor

    Tags

    PuroR, T2A, and Tet-On 3G

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pL-CRISPR.EFS.tRFP

    Plasmid

    #57819

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-tRFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMCS-YFP-GW

    Plasmid

    #209361

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, with YFP tag

    Depositor

    Lucia Strader

    Article

    Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMIA4.721-Keppel

    Plasmid

    #214444

    Purpose

    BxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    2xFKBP12-Casp9
    

    Use

    Hdr donor

    Tags

    P2A-Bsd

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDARMO_HUWE1_3xFLAG

    Plasmid

    #187155

    Purpose

    mammalian cell expression of HUWE1-3xFLAG

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Circular guide cloning vector

    Plasmid

    #180184

    Purpose

    Cloning vector for expressing circular RNA from a U6 promoter

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBHM2679

    Plasmid

    #241724

    Purpose

    Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BSD marker for selection on blasticidin S

    Depositor

    Hiten Madhani

    Article

    Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Sept. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBHM2681

    Plasmid

    #241726

    Purpose

    Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source

    Depositor

    Hiten Madhani

    Article

    Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Sept. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTriEx-NTOM20-LOV2

    Plasmid

    #81009

    Purpose

    Expression of LOV2 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV2
    

    Tags

    NTOM20

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV, p10

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-TRIM28

    Plasmid

    #65397

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

20. GFP-KIF1B beta-FL

    Plasmid

    #79108

    Purpose

    Mammalian expression of GFP-KIF1B beta-FL

    Depositor

    Susanne Schlisio

    Article

    Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

    Insert

    GFP-KIF1B beta full length (KIF1B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    exon 25 missing (natural splice varient)

    Promoter

    CMV

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only