166,778 results

1. pET28a-MH6-Bsu

   Plasmid

   #163911

   Purpose

   Expresses Bsu large fragment for affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   Bsu polymerase
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. RGB-S reporter

   Plasmid

   #207841

   Purpose

   A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli

   Depositor

   Christof Niemeyer

   Article

   Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(

   Inserts

   RpoH sensing construct
   SOS sensing construct
   RpoS sensing construct
   

   Expression

   Bacterial

   Promoter

   PosmY, PsulA, and grpE

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF1932

   Plasmid

   #142292

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   JUN (JUN Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. tol2-mpx-mCherry

   Plasmid

   #29585

   Depositor

   Anna Huttenlocher

   Article

   Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

   Insert

   mpx-mCherry
   

   Use

   Zebrafish expression

   Available Since

   June 3, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pFastBac1-Ac-AChBP

   Plasmid

   #63128

   Purpose

   Expression of Aplysia californica AChBP

   Depositor

   Titia Sixma

   Article

   Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

   Insert

   AChBP
   

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

6. FLHKII-pGFPN3

   Plasmid

   #21920

   Depositor

   Hossein Ardehali

   Article

   Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

   Insert

   Full length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This is the full length human HKII cDNA sequence …

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #191796

   Purpose

   vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized dCas13X
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   R84A, H89A, R739A, R740A, H745A, H746A, H747A

   Promoter

   CBh, SV40, hU6

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CoV2-Spike-D614G

   Plasmid

   #177960

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
   

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAct:dCas9-VPR

   Plasmid

   #78898

   Purpose

   Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   pActin (Drosophila)

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. COVID-SARS2 NSP14/10

    Plasmid

    #159613

    Purpose

    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

    Depositor

    Opher Gileadi

    Article

    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

    Inserts

    Covid-SARS2 Nsp14 (ORF1ab COVID-SARS2, Synthetic)
    Covid-SARS2 Nsp10
    

    Tags

    His6, TEV cleavage site and none

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    (Bicistronic) and T7 - LacO

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBJ053_MTB_jGCaMP7f

    Plasmid

    #209280

    Purpose

    Ubiquitously expresses jGCaMP7f in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

    Depositor

    Adam Cohen

    Article

    Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

    Insert

    jGCaMP7f
    

    Use

    Zebrafish expression, in vitro transcription

    Promoter

    actb2

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lyn11-CIBN-GFP

    Plasmid

    #79572

    Purpose

    expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP

    Depositor

    Pietro De Camilli, Olof Idevall-Hagren

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    Lyn11
    

    Tags

    CIBN (CRY2-binding domain) and EGFP

    Expression

    Mammalian

    Mutation

    G8R (please see depositor comments below)

    Promoter

    CMV

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

13. AMPK(T>A)-SPARK

    Plasmid

    #226256

    Purpose

    phospho-null mutant of AMPK-SPARK

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    AMPK(T>A)-SPARK
    

    Expression

    Mammalian

    Mutation

    substituted Threonine 20 with Alanine

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCL52.2

    Plasmid

    #13656

    Depositor

    Chia Lee

    Article

    Sau et al J Bacteriol. 1997 Mar . 179(5):1614-21.

    Type

    Empty backbone

    Use

    E. coli-s. arueus shuttle vector

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. QPM-sgR (pTaU3)

    Plasmid

    #140448

    Purpose

    For sgRNA expression in monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

    Insert

    TaU3-sgRNA
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    TaU3

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMIA4.721-Olônne

    Plasmid

    #214443

    Purpose

    BxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    2xFKBP12-Casp9
    

    Use

    Hdr donor

    Tags

    P2A-Bsd

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF0612

    Plasmid

    #143054

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RBPJ (RBPJ Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. ClpP-his

    Plasmid

    #22144

    Depositor

    Robert Sauer

    Article

    Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

    Insert

    ClpP-His6
    

    Tags

    his

    Expression

    Bacterial

    Mutation

    C-terminal His tag

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-TRIM28

    Plasmid

    #65397

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pWPT-PDX1

    Plasmid

    #12256

    Depositor

    Didier Trono

    Article

    Ritz-Laser et al Diabetologia. 2003 Jun . 46(6):810-21.

    Insert

    Pdx1 (PDX1 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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