165,187 results

1. mCherry-Moesin-C-14

   Plasmid

   #55103

   Purpose

   Localization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Moesin (MSN Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pGL3-EF1a-i53

   Plasmid

   #216243

   Purpose

   Express i53 in mammalian cells as a HDR enhancer

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   i53
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1hSIRT3_H248Y_HA

   Plasmid

   #24492

   Depositor

   Eric Verdin

   Article

   Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

   Insert

   Human Sirtuin 3 catalytically inactive mutant (SIRT3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H248Y

   Available Since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF5B-FRT-cilia-APEX

   Plasmid

   #73186

   Purpose

   Expresses cilia-APEX in mammalian cells

   Depositor

   Maxence Nachury

   Article

   Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

   Insert

   cilia-APEX (Nphp3 Glycine max, Mouse)
   

   Tags

   internal GFP tag

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF2364

   Plasmid

   #142051

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CREB1 (CREB1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

   Plasmid

   #113888

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL-CRISPR.SFFV.GFP

   Plasmid

   #57827

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   SFFV
   P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. YIplac211-yoHaloTag-TLG1

   Plasmid

   #105277

   Purpose

   Plasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Tlg1 (TLG1 Budding Yeast)
   

   Tags

   yoHaloTag

   Expression

   Yeast

   Mutation

   HaloTag has yeast optimized codons

   Available Since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRBad-Z-CspGFP

   Plasmid

   #40730

   Depositor

   Brian McNaughton

   Article

   Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

   Insert

   Leucine Zipper prime - C super positive Green Fluorescent Protein
   

   Expression

   Bacterial

   Promoter

   pBad

   Available Since

   Nov. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. TRIPZ-bioGEF-UBwt-PURO

    Plasmid

    #208045

    Purpose

    Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    Ub (UBC Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG/ATP8B1-HA

    Plasmid

    #209209

    Purpose

    Mammalian expression of ATP8B1

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP8B1 (ATP8B1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223-TGFBR2

    Plasmid

    #23623

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TGFBR2 (TGFBR2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pExp-Sumo

    Plasmid

    #112574

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8His and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. EB3-mNeonGreen

    Plasmid

    #98881

    Purpose

    In vivo visualization of microtuble plus ends (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    EB3
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.3-SARS2-B.1.617.2

    Plasmid

    #172320

    Purpose

    expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike of B.1.617.2 strain (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

    Promoter

    CMV-F

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3_cAMPFIRE-H

    Plasmid

    #182281

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-H (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CRISPR-SP-Cas9 reporter

    Plasmid

    #62733

    Purpose

    Mammalian CRISPR-SP-Cas9 reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    CRISPR-SP-Cas9 target seq + tdTomato (out of frame)
    

    Use

    CRISPR and Lentiviral

    Tags

    CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

    Expression

    Mammalian

    Promoter

    EF1-Alpha

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRRL.PPT.SFFV.HA.RUNX1C.IRES.eGFP

    Plasmid

    #181980

    Purpose

    Human RUNX1C gene overexpression

    Depositor

    Jan-Henning Klusmann

    Insert

    RUNX1C (human) (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    HA (Human influenza hemagglutinin)

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-ASAP3ER

    Plasmid

    #195356

    Purpose

    ASAP3ER under the control of the pCAG promoter

    Depositor

    Brenda Bloodgood

    Article

    Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

    Insert

    ASAP3ER
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-Flag-c-Myc

    Plasmid

    #102626

    Purpose

    Expresses c-Myc in mammalian cells

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    MYC proto-oncogene, bHLH transcription factor (MYC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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