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p3xFLAG CMV14 / BRAF-V600E

Plasmid

#131723

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

V600E

Promoter

CMV

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

Plasmid

#128424

Purpose

Human Codon Optimized dCas9-DNMT3A3L

Depositor

Bradley Bernstein

Article

Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

Insert

dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

T7 Promoter

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pci-EGFP-UPF1-WT

Plasmid

#135994

Purpose

WT UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:CoChR-tdTomato

Plasmid

#124233

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

CoChR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:Renilla

Plasmid

#118066

Purpose

Transcriptional unit for constitutive expression of Renilla in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:GreenRenilla:bGHF

Use

Luciferase and Synthetic Biology; Assembly of gb2…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6Xhis-human SUMO 3

Plasmid

#133772

Purpose

express 6Xhis- SUMO 3 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 3 (SUMO3 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry-CaM

Plasmid

#127393

Purpose

Fluorescent reporter for calmodulin 1

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

Calmodulin 1 (Calm1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

pCAG

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET-QUE2m

Plasmid

#129350

Purpose

ATP measurement

Depositor

Hiroyuki Noji

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-2m

Expression

Bacterial

Available Since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pUCC001

Plasmid

#124451

Purpose

Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

HH-BsaI

Expression

Yeast

Mutation

gRNA expression cassette from pUDP002 modified to…

Promoter

TDH3 promoter from S.cerevisiae (already present …

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-miniTurbo-L2

Plasmid

#127352

Purpose

gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130995

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Promoter

hSyn

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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spisPink chromoprotein

Plasmid

#117839

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, spisPink

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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CROPseq-Guide-Zeo

Plasmid

#127173

Purpose

Entry plasmid for guide cloning

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCFD3.1-w-dU6:3gRNA

Plasmid

#123366

Purpose

gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

Depositor

Simon Bullock

Insert

U6:3-gRNA

Use

CRISPR

Expression

Insect

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag VP64 nog

Plasmid

#120251

Purpose

CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-COX4-dAPEX2

Plasmid

#117177

Purpose

Plasmid name in publication: pAAV-DIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

COX4-dAPEX2

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR3

Plasmid

#116742

Purpose

Lentiviral expression of FGFR3

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR3 (FGFR3 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA-Sirius-4X(MS2-PP7)

Plasmid

#121941

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-4X(MS2-PP7)

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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SynTagMA_pre

Plasmid

#119738

Purpose

Synaptic Tag for Mapping Activity

Depositor

Thomas Oertner

Article

Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

Insert

SynTagMA_pre

Use

AAV

Expression

Mammalian

Promoter

hsyn1

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1801_pmax-dCasRx

Plasmid

#118634

Purpose

Transient transfection; Expresses dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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