pLVX_TRE3G_DD::mCherry
(Plasmid #108679)

• Purpose: Tunable system for controlled gene expression and protein stability in mammalian cells
• Depositing Lab: Lucia Marucci
• Publication: Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi: 10.1038/s41467-019-12329-9.

Backbone

• Vector backbone: pLVX
• Backbone size w/o insert (bp): 7849
• Total vector size (bp): 9044
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Destabilising Doman (DD) mCherry fusion protein
• Alt name: DD::mCherry
• Insert Size (bp): 1267
• Promoter: Doxycycline inducible
• Tag / Fusion Protein:
  • DD::mCherry fusion protein (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGCGGCCGCGGATCCCCCGGGCCACATGATCAGCCTGATCGCCGC
• 3′ sequencing primer: GCTTCTCGAGGAATTCCCCGGGTTACTTGTACAGCTCGTCCATGC

Resource Information

• Supplemental Documents:
  • pLVX TET3G dd mCherry.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

These Materials were created under an MRC grant. Any publications which include use of these materials must acknowledge the MRC as follows: "This work was supported by the Medical Research Council grant number MR/N021444/1."