Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins.
Ast C, Foret J, Oltrogge LM, De Michele R, Kleist TJ, Ho CH, Frommer WB
Nat Commun. 2017 Sep 5;8(1):431. doi: 10.1038/s41467-017-00400-2.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100017 | pET15b-eGO-Matryoshka-LE-LP | Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers |
| 100018 | pET15b-sfGO-Matryoshka-LE-LP | Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers |
| 100019 | pRSET a MatryoshCaMP6s | Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP. |
| 100020 | pRSET a sfMatryoshCaMP6s | Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP. |
| 100021 | pRSET a sfMatryoshCaMP6s-T78H | Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H |
| 100022 | pRSET a sfGCaMP6s | Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant. |
| 100023 | pRSET a sfGCaMP6s-T78H | Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation |
| 100024 | pGPTVII-Bar-U-MatryoshCaMP6s | Plant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP. |
| 100025 | pcDNA3.2-DEST-MatryoshCaMP6s | Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP. |
| 100922 | pDRF'- AmTryoshka1;3-LS-F138I | Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP. |
| 100963 | pDRF'- AmTryoshka1;3-LS-F138I-T78H | Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP |
| 100964 | pDRF'- AmTryoshka1;3-LS-L255I | Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP. |
| 100965 | pDRF'- AmTryoshka1;3-GS-F138I | Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP. |
| 100966 | pDRF'- AmTryoshka1;3-GS-L255I | Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP. |
| 100967 | pDRF’- AmTryoshka1;3-GS | Yeast expression of control vector for fluorescent sensors reporting the activity of ammonium transceptors. |