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Addgene

pcDNA3.2-DEST-MatryoshCaMP6s
(Plasmid #100025)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100025 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.2/V5-DEST
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 5490
  • Total vector size (bp) 7449
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MatryoshCaMP6s
  • Alt name
    GCaMP6s-LSSmOrange
  • Alt name
    MCaMP6s
  • Insert Size (bp)
    1959
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 Epitope Tag (C terminal on backbone)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The GCaMP6s portion was derived from Addgene plasmid #40753 (Douglas Kim).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.2-DEST-MatryoshCaMP6s was a gift from Wolf Frommer (Addgene plasmid # 100025 ; http://n2t.net/addgene:100025 ; RRID:Addgene_100025)
  • For your References section:

    Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins. Ast C, Foret J, Oltrogge LM, De Michele R, Kleist TJ, Ho CH, Frommer WB. Nat Commun. 2017 Sep 5;8(1):431. doi: 10.1038/s41467-017-00400-2. 10.1038/s41467-017-00400-2 [pii] PubMed 28874729