Purposelentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85144||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8785
- Total vector size (bp) 12398
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameCDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3
SpeciesH. sapiens (human)
Insert Size (bp)3114
Entrez GeneCDH5 (a.k.a. 7B4, CD144)
- Promoter CAG
/ Fusion Protein
- mRuby3 (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer GTTATTGTGCTGTCTCATC
- 3′ sequencing primer ACACCGGCCTTATTCCAA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Remark on cloning and propagating of cDNA encoding human CDH5: In our experience, CDH5 cDNA was toxic to bacteria when cloned into CMV-driven expression plasmids, resulting in small or mutated clones.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLV-CDH5-mRuby3-IRES-puro was a gift from Tobias Meyer (Addgene plasmid # 85144 ; http://n2t.net/addgene:85144 ; RRID:Addgene_85144)
For your References section:Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells. Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, Betzig E, Meyer T. Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub 2016 Nov 14. 10.1038/ncb3438 PubMed 27842057