Purposelentiviral expression of a stoichiometric F-actin and myosin light chain reporter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85146||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8614
- Total vector size (bp) 10754
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)2169
- Promoter EF1a
/ Fusion Protein
- mRuby3, mTurquoise
- Cloning method Gibson Cloning
- 5′ sequencing primer TCAAGCCTCAGACAGTGGTTC
- 3′ sequencing primer ACACCGGCCTTATTCCAA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Stoichiometric expression of Ftractin-mRuby3 and mTurquiose-MLC is achieved through insertion of a ribosomal skipping sequence (P2A) between the two reporter sequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast was a gift from Tobias Meyer (Addgene plasmid # 85146 ; http://n2t.net/addgene:85146 ; RRID:Addgene_85146)
For your References section:Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells. Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, Betzig E, Meyer T. Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub 2016 Nov 14. 10.1038/ncb3438 PubMed 27842057