168,786 results

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pEGFP-N1 human cofilin WT

Plasmid

#50859

Purpose

expresses human cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pLP-mScarlet-I

Plasmid

#209993

Purpose

Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

mScarlet-I

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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Lck-GFP

Plasmid

#61099

Purpose

Expression of a membrane-targeted form of eGFP

Depositor

Steven Green

Article

Benediktsson et al J Neurosci Methods. 2005 Jan 30;141(1):4

Insert

Lck-GFP

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

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DD-DamV133A-LMNB1-IRES2-mCherry

Plasmid

#159599

Purpose

Shield-1 inducible transient mammalian expression of Dam-LMNB1 for DamID, containing the V133A mutant of Dam.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamV133A-LMNB1-IRES2-mCherry

Expression

Mammalian

Mutation

Dam V133A mutation

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 puro

Plasmid

#104994

Purpose

This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CD80

Plasmid

#223603

Purpose

Lentiviral expression of human CD80 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-HLA-G SCT-T2A-GFP

Plasmid

#205462

Purpose

lentiviral plasmid for expression of HLA-G SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

HLA-G SCT (HLA-G Human)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_PLK1_Pkinase

Plasmid

#109933

Purpose

Protein expression and purification of PLK1_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PLK1_Pkinase

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-HA PIKFYVE K1831E

Plasmid

#245055

Purpose

Kinase-dead mutant

Depositor

Thomas Martin

Insert

PIKFYVE (PIKFYVE Human)

Tags

EGFP-HA

Expression

Mammalian

Mutation

K1831E

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-N LMP2A

Plasmid

#37963

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

LMP2A (LMP-2A H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV6M-Pak1

Plasmid

#12209

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-2-EWSR1

Plasmid

#46384

Depositor

Heinz Gehring

Article

Pahlich et al Proteins. 2005 Oct 1;61(1):164-75.

Insert

EWSR1 (EWSR1 Human)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-N-Flag-NLRP3 1-90

Plasmid

#75137

Purpose

Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 1-90

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-SOX4

Plasmid

#110360

Purpose

Mammalian expression of FLAG-tagged SOX4

Depositor

Carlos Moreno

Article

Liu et al Cancer Res. 2006 Apr 15;66(8):4011-9. do

Insert

SOX4 (SOX4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

lacking 5' UTR

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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CaV2.1 (pcDNA6)

Plasmid

#26578

Depositor

Diane Lipscombe

Article

Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

Insert

Cacna1a (Cacna1a Mouse)

Tags

His6 and V5

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pCW57.1 CCNE1

Plasmid

#164144

Purpose

Doxycycline inducible expression of Cyclin E1

Depositor

Richard Possemato

Insert

CCNE1 (CCNE1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2021

Availability

Academic Institutions and Nonprofits only

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EBFP2-PCNA-19-SV40NLS-4

Plasmid

#55250

Purpose

Localization: Nucleus, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

3xSV40 NLS and EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pH-A3A-PBE

Plasmid

#119774

Purpose

Conversion of C to T in rice plants transformation

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS-UGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

maize Ubiquitin-1

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(0)

Plasmid

#199166

Purpose

used to express a neutral GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(0)

Tags

MGHHHHHHGGA

Mutation

E16R, I138R, D207R, N222K, L246R

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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