179,354 results
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Plasmid#92065PurposeModified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).DepositorInsertMS2-P65-HSF1_2A_Neo (HSF1 Human, Synthetic)
UseLentiviralExpressionMammalianMutationN55K in MS2PromoterEF1aAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pl-Synapsin-YFP-NaVII-III
Plasmid#91246PurposeExpresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)DepositorInsertNav1.2 (Scn2a Rat)
UseLentiviralTagsYFPExpressionMammalianMutationThe insert codes only for the intracellular loop …Available sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmCherry1
Plasmid#89986PurposeLong stokes-shift variant of red fluorescent protein mCherryDepositorInsertLSSmCherry1
Tags6xHis-T7-XpressExpressionBacterialAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCK302
Plasmid#87768PurposeAs pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be usedDepositorInsertsPrhaBAD-sfGFP
rhaS from E. coli with its native RBS from E. coli
UseSynthetic BiologyTags6xHis TagExpressionBacterialPromoterPrhaBAD rhamnose-inducible promoter from E. coli …Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPT107
Plasmid#85525PurposeRepressilator with triple reporterDepositorInsertsmCfp
mVenus
tetR
lacI
cI
mKate2
UseSynthetic BiologyTagsssrAExpressionBacterialAvailable sinceApril 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AICSDP-13:FBL-mEGFP
Plasmid#87427PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human FBLDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFBL Homology Arms with linker-mEGFP (FBL Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pLenti-V5-KDM3A
Plasmid#82331Purposelentiviral expression of KDM3ADepositorInsertKDM3A (KDM3A Human)
UseLentiviralTagsV5ExpressionMammalianMutationtwo silent mutations to make it siRNA resistantAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFG2
Plasmid#87250Purposegene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-NatDepositorInsertNat
TagsparS segmentsExpressionYeastPromoterTEFAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-CB-iCre-P2A-tdTomato-T2A-Puro
Plasmid#72255PurposeExpress iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoterDepositorInsertiCre-P2A-tdTomato-T2A-Puro
UseLentiviralExpressionMammalianPromoterCB and chicken beta-actin + CMV enhancerAvailable sinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRH2502
Plasmid#84379PurposeExpression of dcas9 D10A H840A from a TetR-regulated uvtetO promoterDepositorInsertdcas9
UseCRISPRExpressionBacterialMutationAspartate at position 10 changed to Alanine, Hist…Promoteruv15tetOAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-RSV-SpCas9
Plasmid#85450PurposeExpress SpCas9 in mammalian cellsDepositorHas serviceAAV8InsertpRSV
UseAAVPromoterpRSVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LeGO-mOrange2
Plasmid#85212PurposeLentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoterDepositorInsertmOrange2
UseCre/Lox and LentiviralExpressionMammalianPromoterSFFVAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_AXP_hrGFP
Plasmid#84613PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locusDepositorPublicationInserthrGFP
UseSynthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV2.5-THP-dsRed2
Plasmid#80335PurposeFluorescent Reporter for Dopaminergic Neuron DifferentiationDepositorAvailable sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21
Plasmid#83421PurposeMouse Rab21 (wild type)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab21 (Rab21 Mouse)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV_Zika_NS2B_Flag
Plasmid#79637PurposeThird generation Self-inactivating lentivector expressing NS2B from Zika virusDepositorInsertNS2B
UseLentiviralTagsFlagExpressionMammalianPromoterEF1Available sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR
Plasmid#82024PurposeLentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selectionDepositorPublicationInsertmKate2-2xNLS
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV10-3xFlag Skp2 WT
Plasmid#81115Purposemammalian expression of Skp2 WTDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSKP2 (SKP2 Human)
Tags3xFlagExpressionMammalianPromoterCMVAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX4T-hLIG3
Plasmid#81055Purposebacterial expression of human LIG3DepositorAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET31b-T7-Spinach2
Plasmid#79783PurposeExpresses Spinach2 under a T7 promoterDepositorInsertT7-tRNA-Spinach2
ExpressionBacterialPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by