166,778 results

1. pMIA3-Keppel

   Plasmid

   #214446

   Purpose

   Expresses eSpCas9 and gRNA targeting Keppel-19 GSH

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   eSpCas9-P2A-mRuby2-P2A-tp53dn
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDARMO_HUWE1_3xFLAG

   Plasmid

   #187155

   Purpose

   mammalian cell expression of HUWE1-3xFLAG

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLX1.8NFATGFPpd2HS4

   Plasmid

   #169096

   Purpose

   Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation

   Depositor

   Geoffrey Owens

   Inserts

   NFAT enhancer (from IL-4 promoter)
   Destabilized green fluorescent protein
   

   Use

   Lentiviral

   Promoter

   NFAT enhancer + synthetic TATA box

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-NP

   Plasmid

   #74737

   Purpose

   mammalian expression of the Nucleoprotein of the A/New Caledonia/20/99 (H1N1) Influenza virus

   Depositor

   Elisa Vicenzi

   Article

   Di Pietro et al J Virol. 2013 Apr;87(8):4523-33. doi: 10

   Insert

   Nucleoprotein
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-BBS1

   Plasmid

   #218711

   Purpose

   Expresses N-terminally EGFP-tagged BBS1 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Insert

   BBS1 (BBS1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM6-HpaBC

   Plasmid

   #71260

   Purpose

   Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6

   Depositor

   Mattheos Koffas

   Article

   Jones et al Biotechnol Prog. 2015 Oct 21. doi: 10.10

   Inserts

   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mTurquoise2-MapTau

   Plasmid

   #137807

   Purpose

   Visualization of microtubules

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   MapTau
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pL-CRISPR.EFS.tRFP

   Plasmid

   #57819

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3/HA-SUMO3 (Sentrin 2)

   Plasmid

   #17361

   Purpose

   Mammalian expression of human SUMO3 with HA tag

   Depositor

   Edward Yeh

   Article

   Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

   Insert

   SUMO3 (SUMO3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJC5-LAMP1-RFP-2xFlag

    Plasmid

    #102931

    Purpose

    Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    LAMP1
    

    Use

    Lentiviral

    Tags

    mRFP-2xFlag

    Promoter

    ubc

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. TDP43-NTD

    Plasmid

    #241024

    Purpose

    Bacterial expression of TDP-43 NTD

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP43-NTD
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    changed N81 to stop codon

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-GRAB_SST1.0

    Plasmid

    #208665

    Purpose

    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cas9 TLS2

    Plasmid

    #196978

    Purpose

    Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Cas9 with TLS2 tag
    

    Expression

    Plant

    Promoter

    pG10-90

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAX APP 695

    Plasmid

    #30137

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Expression

    Mammalian

    Mutation

    wild type splice variant 695

    Available Since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. ND4.2-Left TALE-G1397-N-DddA11-mCherry

    Plasmid

    #179682

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
    

    Expression

    Mammalian

    Mutation

    S1330I A1341V N1342S E1370K T1380I

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLacO-ISce1

    Plasmid

    #58505

    Purpose

    provides 256 Lac operator repeats adjacent to the ISce1 restriction site

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Lac repeats adjacent to 6 copies of the ISce1 restriction site
    

    Expression

    Bacterial

    Promoter

    no promoter

    Available Since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO-mito-mCer

    Plasmid

    #185602

    Purpose

    Lentiviral construct for expression of mCerulean targeted to mitochondria

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    mito-mCer
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)

    Plasmid

    #191389

    Purpose

    Donor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hCdt1(1-100)
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMAL-p2X

    Plasmid

    #75287

    Purpose

    Expresses proteins in the periplasm as fusions to maltose-binding protein

    Depositor

    Paul Riggs

    Article

    Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

    Type

    Empty backbone

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 18, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pBP Omomer

    Plasmid

    #113169

    Purpose

    Retroviral vector for expressing Omomer (tamoxifen inducible Omomyc), by DNA transfection or retroviral infection.

    Depositor

    Sergio Nasi

    Article

    Soucek et al Cancer Res. 2002 Jun 15;62(12):3507-10.

    Insert

    Omomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    E57T, E64I, R70Q, R71N

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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