165,187 results

1. pCDNA3 Flag p38 alpha

   Plasmid

   #20351

   Depositor

   Roger Davis

   Article

   Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

   Insert

   pDCNA3 Flag p38 alpha (Mapk14 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2xSTAT6-Luc2P

   Plasmid

   #35486

   Depositor

   Axel Nohturfft

   Insert

   STAT6 promoter
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   2x Stat6-c/EBP

   Available Since

   March 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD

   Plasmid

   #197687

   Purpose

   plasmid for expressing the DNMT3bCD

   Depositor

   Michael Elowitz

   Article

   Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

   Insert

   H2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Synthetic, Human)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAB076i3

   Plasmid

   #79198

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available Since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. hCMV-IE1:FLuc

   Plasmid

   #118063

   Purpose

   Transcriptional unit for constitutive expression of FLuc in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:FLuc:bGHF
   

   Use

   Luciferase and Synthetic Biology; Assembly of gb2…

   Expression

   Mammalian

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-teLuc c-myc

   Plasmid

   #100026

   Purpose

   Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

   Depositor

   Huiwang Ai

   Article

   Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

   Insert

   teLuc, a NanoLuc mutant
   

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   D19S, D85N, C164H

   Promoter

   CMV

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET51b(+)_EGFP_NbALFA

   Plasmid

   #136626

   Purpose

   Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-NbALFA
   

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHBS1501 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaRRM]

   Plasmid

   #133325

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of CTD in full-length TDP43

   Available Since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHCA/GAL4(1-93).ER.VP16

   Plasmid

   #108216

   Purpose

   Yeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domain

   Depositor

   Didier Picard

   Article

   Louvion et al Gene 1993 Sep 6;131(1):129-34.

   Insert

   Gal4 DBD-ERalpha HBD-VP16
   

   Expression

   Yeast

   Mutation

   ERalpha HBD has G400V mutation

   Promoter

   ADH1

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV 3xFLAG-GABARAP WT

    Plasmid

    #123097

    Purpose

    Expresses 3xFLAG-GABARAP wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-parkin C431S

    Plasmid

    #45877

    Depositor

    Edward Fon

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin (PRKN Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C431S mutation (ubiquitin acceptor site)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pMRXIP VPS16-GFP

    Plasmid

    #67023

    Purpose

    Human VPS16 ORF was inserted into pMRXIP GFP-N vector

    Depositor

    Noboru Mizushima

    Article

    Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

    Insert

    Homo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (VPS16 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Promoter

    CMV

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

13. CHRM2-Tango

    Plasmid

    #66249

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CHRM2 (CHRM2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-PLE-IRES-mCherry

    Plasmid

    #45766

    Depositor

    Luke Lavis

    Article

    Tian et al Proc Natl Acad Sci U S A. 2012 Mar 27;10

    Insert

    esterase
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC-CANTHipi

    Plasmid

    #53301

    Purpose

    Contains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtO
    

    Use

    Low copy number bacterial cloning vector

    Tags

    6His

    Promoter

    Trc

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221 msNLRP3

    Plasmid

    #218639

    Purpose

    Gateway shuttling vector for cloning C-terminal NLRP3 tags (contains no stop codon).

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Gateway entry

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 12.4kbVillin-ΔATG

    Plasmid

    #19358

    Depositor

    Deborah Gumucio

    Article

    Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

    Insert

    12.4kb Villin promoter
    

    Expression

    Bacterial

    Mutation

    No ATG - transgenes cloned downstream of the vill…

    Available Since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHA-eIF4E

    Plasmid

    #17343

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    eIF4E (EIF4E Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-Sec23A

    Plasmid

    #66609

    Purpose

    GFP fusion of Sec23A

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec23A (SEC23A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. Stx5L-mCherry

    Plasmid

    #209844

    Purpose

    Expresses Stx5L-mCherry in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    STX5 (STX5 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Wildtype Stx5 (with 55M)

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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