168,786 results

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PB-TAC-ERP2-(N-HA)KRAS_G12D

Plasmid

#201254

Purpose

Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

Depositor

Alexander Kleger

Article

Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

Insert

KRAS (KRAS Human)

Use

Piggybac transposon

Tags

HA-Tag

Expression

Mammalian

Mutation

changed Gly (G) 12 to Asp (D)

Promoter

TRE

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGMCK-T38S38-750-luxAB-DAS+4

Plasmid

#49518

Purpose

Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

T38S8-P750-luxAB-DAS+4

Tags

DAS+4 (AANDENYSENYADAS)

Expression

Bacterial

Promoter

P750

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Plasmid

#132543

Purpose

Expresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Tags

FLAG tag

Expression

Mammalian

Promoter

cmv d0

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pU6_N7-sgRNA2

Plasmid

#190664

Purpose

Expresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloning

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

N7 sgRNA2

Expression

Mammalian

Promoter

U6

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAD-sfGFP(Sp)-Pman

Plasmid

#133833

Purpose

Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

Depositor

Oscar Kuipers

Article

Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

Insert

sfGFP(Sp)

Use

E.coli/bacillus spp. shuttle vector

Expression

Bacterial

Promoter

Pman

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-LACTB-dAPEX2

Plasmid

#117179

Purpose

Plasmid name in publication: pAAV-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

LACTB-dAPEX2

Use

AAV

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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Myc-TEAD4

Plasmid

#24638

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD-4 (TEAD4 Human)

Tags

Myc

Expression

Mammalian

Available Since

May 13, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3B WT

Plasmid

#123092

Purpose

Expresses 3xFLAG-LC3B wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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A*11:01:01

Plasmid

#203281

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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mito-meGFP

Plasmid

#172481

Purpose

meGFP targeted to the mitochondrial matrix with a human COX8 presequence

Depositor

Thomas Schwarz

Insert

Mito

Tags

meGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Red cGull

Plasmid

#191853

Purpose

Expresses biosensor for cGMP in mammalian cells

Depositor

Takashi Tsuboi

Article

Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

Insert

Red cGull

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-cfGFP

Plasmid

#200773

Purpose

Expresses cysteine-free GFP (cfGFP) under synapsin promoter

Depositor

Alexander Dityatev

Article

Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

Has Service

AAV8

Insert

cysteine-free GFP

Use

AAV

Promoter

Synapsin

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42-Q61L

Plasmid

#12986

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 constitutively active (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q61L Constitutively active

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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Halo-Rab6A

Plasmid

#190171

Purpose

Expresses HaloTag-Rab6A in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

HaloTag-Rab6A

Tags

HaloTag

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-hChR2(H134R)-EYFP

Plasmid

#26973

Purpose

AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation

Depositor

Karl Deisseroth

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

Insert

hChR2(H134R)

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Promoter

hSyn

Available Since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 N

Plasmid

#158079

Purpose

Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 nucleocapsid (N )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CIp10 - γmGFP (γmGFP based)

Plasmid

#163119

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

gamma mGFP

Use

Candida albicans expression

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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LeGO-V2

Plasmid

#27340

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, Venus fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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CMV HaloTag-LgBiT Vector

Plasmid

#236917

Purpose

Mammalian expression vector encoding HaloTag(R)-LgBiT for use in HiBiT cell lines

Depositor

Promega Corporation

Has Service

DNA

Type

Empty backbone

Tags

HaloTag (R)-LgBiT

Expression

Mammalian

Mutation

None

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pME-mCherry-FLAG-CD59-GPI

Plasmid

#50378

Purpose

Express mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian cells

Depositor

Reika Watanabe

Article

Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

Insert

mCherry

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only