161,152 results

1. EGFP-PIP5K1A

   Plasmid

   #202720

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   EGFP:PIP5K1A (PIP5K1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMLS7-egfp

   Plasmid

   #112917

   Purpose

   Fluorescence microscopy

   Depositor

   Miguel Valvano

   Article

   Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PS7 promoter and Ps7

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-TetOne-Puro-mitoLbNOX-FLAG

   Plasmid

   #218667

   Purpose

   pLVX-TetOne-Puro-mitoLbNOX-FLAG

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   mitoLbNOX
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG and MTS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCE35

   Plasmid

   #174409

   Purpose

   C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.

   Depositor

   Clarissa Nobile

   Article

   Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

   Insert

   C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. NSK021 CMV-TO-p53R248Q (FLP-IN)

   Plasmid

   #191167

   Purpose

   Expression of human p53 mutant R248Q in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   p53(R248Q) (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R248Q

   Promoter

   CMV-TO

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJYS1Ptac

   Plasmid

   #85545

   Purpose

   Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   recT
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac and unknown

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas1+2

   Plasmid

   #72676

   Purpose

   Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

   Depositor

   Udi Qimron

   Article

   Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

   Insert

   Cas1, Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-Ki67-mCherry

   Plasmid

   #183748

   Purpose

   Expresses EGFP-Ki67-mCherry

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 (MKI67 Human)
   

   Use

   Tags

   Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV SYN flex PSAM4 GlyR IRES EGFP

   Plasmid

   #119741

   Purpose

   Cre-dependent chemogenetic inhibitor expression

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Has Service

   AAV5 and AAV9

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   synapsin

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. piggyBAC-BRD4-Flag

    Plasmid

    #214432

    Purpose

    A piggyBAC based plasmid for the stable expression of BRD4 with a flag tag.

    Depositor

    Shaun Cowley

    Insert

    Brd4 bromodomain containing 4 (Brd4 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. DT480

    Plasmid

    #80413

    Purpose

    Expresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a-CysRS-His

    Plasmid

    #124107

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Cysteinyl-tRNA ligase (cysS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCherry-EGFP

    Plasmid

    #86639

    Purpose

    Expresses mCherry-eGFP tandem (cytoplasmic)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    mCherry-EGFP
    

    Use

    Tags

    mCherry-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-H2B-AID-EYFP

    Plasmid

    #47329

    Purpose

    for plasmid integration using the Flp-In system. AID at C-terminal

    Depositor

    Don Cleveland

    Article

    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

    Insert

    histone H2B (H2BC21 Human)
    

    Use

    Tags

    AID and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIS2

    Plasmid

    #12177

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDUAL CD81 (GFP)

    Plasmid

    #86980

    Purpose

    Lentiviral expression construct encoding CD81 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    CD81 (CD81 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. 10TALE_Pmin_fLuc

    Plasmid

    #160663

    Purpose

    Expression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domain

    Depositor

    Roman Jerala

    Article

    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

    Insert

    10TALE_Pmin_fLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMy-BirA-T2A-eGFP

    Plasmid

    #50565

    Purpose

    Retroviral vector encoding for GFP and the biotinylating enzyme BirA

    Depositor

    Rafael Casellas

    Article

    Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

    Insert

    Ampicilin
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. gCH128 (non-targeting crRNA)

    Plasmid

    #217339

    Purpose

    Non-targeting crRNA

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    crNT-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-FRT/TO-3*Flag-APEX2 N-term COILIN

    Plasmid

    #187581

    Purpose

    Express FLAG epitope and APEX2-tagged COILIN fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    COILIN (COIL Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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