EGFP-DCX
(Plasmid #32852)

• Depositing Lab: Joseph Gleeson
• Publication: Tanaka et al J Cell Biol. 2004 Jun 7. 165(5):709-21.

Backbone

• Vector backbone: pEGFP-C
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DCX
• Alt name: doublecortin
• Species: H. sapiens (human)
• Insert Size (bp): 1110
• Entrez Gene: DCX (a.k.a. DBCN, DC, LISX, SCLH, XLIS)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: see map (unknown if destroyed)
• 3′ cloning site: see map (unknown if destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • GFP-DCX map, cutsites and sequence
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the full plasmid sequence provided has been converted from paper format and may differ from the sequencing results obtained by Addgene. These differences should not affect the function of the plasmid.