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pCMMP-LMP1-IRES-eGFP
(Plasmid #36955)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36955 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMMP-MCS-IRES-eGFP
  • Backbone manufacturer
    Addgene plasmid #36953
  • Backbone size w/o insert (bp) 7037
  • Total vector size (bp) 8495
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LMP1
  • Alt name
    latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV)
  • Entrez Gene
    LMP-1 (a.k.a. HHV4_LMP-1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer pLXSN_5
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sugden lab plasmid p3455

Please note that the full plasmid sequence provided by the depositing laboratory and Addgene's sequencing results differ from GenBank entry YP_401722.1 by three polymorphisms: F106Y, I122L and M129I. These differences result from the particular EBV isolate. For more information please see the following article: https://www.ncbi.nlm.nih.gov/pubmed/7931160

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMMP-LMP1-IRES-eGFP was a gift from Bill Sugden (Addgene plasmid # 36955 ; http://n2t.net/addgene:36955 ; RRID:Addgene_36955)
  • For your References section:

    The Latent Membrane Protein 1 (LMP1) Oncogene of Epstein-Barr Virus Can Simultaneously Induce and Inhibit Apoptosis in B Cells. Pratt ZL, Zhang J, Sugden B. J Virol. 2012 Apr;86(8):4380-93. Epub 2012 Feb 8. 10.1128/JVI.06966-11 PubMed 22318153