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Cre Stoplight
(Plasmid #45150)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45150 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogene
  • Backbone size w/o insert (bp) 3342
  • Total vector size (bp) 5387
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cre Stoplight
  • Alt name
    Original Stoplight
  • Insert Size (bp)
    2045
  • Promoter CMV
  • Tags / Fusion Proteins
    • DsRed (N terminal on insert)
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site refer to map (unknown if destroyed)
  • 3′ cloning site refer to map (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cre Stoplight was a gift from Thomas Hughes (Addgene plasmid # 45150 ; http://n2t.net/addgene:45150 ; RRID:Addgene_45150)
  • For your References section:

    Cre stoplight: a red/green fluorescent reporter of Cre recombinase expression in living cells. Yang YS, Hughes TE. Biotechniques. 2001 Nov;31(5):1036, 1038, 1040-1. 10.2144/01315st03 PubMed 11730010