166,806 results

1. pLentiPGK Puro DEST p38KTRClover

   Plasmid

   #59152

   Purpose

   Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-PM-ExRai-AktAR2

   Plasmid

   #184050

   Purpose

   Plasma membrane-targeted ExRai-AktAR2

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   PM-ExRai-AktAR2
   

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV 3xFLAG-ATG3 WT

   Plasmid

   #129296

   Purpose

   Expresses 3xFLAG-ATG3 WT in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. LD-B

   Plasmid

   #209861

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Tags

   Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDonor_ShCAST_kanR

   Plasmid

   #127924

   Purpose

   Kanamycin resistance donor for ShCAST

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   KanR, R6K origin of replication
   

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Flag-hGSDME

   Plasmid

   #218939

   Purpose

   Expression of N-terminal FLAG tagged human gasdermin E

   Depositor

   Christopher LaRock

   Article

   LaRock et al Nature. 2022 May;605(7910):527-531. doi:

   Insert

   human GSDME (GSDME Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. COVID-SARS2 NSP14/10

   Plasmid

   #159613

   Purpose

   Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

   Depositor

   Opher Gileadi

   Article

   Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

   Inserts

   Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
   Covid-SARS2 Nsp10
   

   Tags

   His6, TEV cleavage site and none

   Expression

   Bacterial

   Mutation

   Codon-optimized for E. coli expression

   Promoter

   (Bicistronic) and T7 - LacO

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAF0690 Ef1a-Kp03_attB-BFP acceptor

   Plasmid

   #193473

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Kp03_attB
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19_CRISPR_DpmrA

   Plasmid

   #160903

   Purpose

   CRISPR_Cas9, lambda red recombineering

   Depositor

   Anne-Catrin Uhlemann

   Article

   McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

   Inserts

   Cas9
   Lambda Red Recombineering Genes
   Zeocin Resistance Cassette
   

   Promoter

   araBAD

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GAPDH-CPG-TV

    Plasmid

    #70148

    Purpose

    a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

    Depositor

    Rudolf Jaenisch

    Article

    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

    Insert

    5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
    

    Use

    Mouse Targeting and Synthetic Biology

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCVpuro-Cre

    Plasmid

    #34564

    Purpose

    Retroviral expression of Cre

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Expression

    Mammalian

    Promoter

    5'LTR

    Available Since

    Jan. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-FLAG-LOV*-H2B

    Plasmid

    #202204

    Purpose

    Expresses the photoproximity labeling catalyst LOV* fused to H2B in mammalian cells

    Depositor

    Thomas Muir

    Article

    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    Histone H2B type 1-K
    

    Tags

    FLAG and LOV*

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-EGFP-C-IFT81

    Plasmid

    #218732

    Purpose

    Expresses N-terminally EGFP-tagged IFT81 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

    Insert

    IFT81 (IFT81 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AiP12258 - pAAV-AiE0479m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2258)

    Plasmid

    #230107

    Purpose

    AiE0479m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO APEX2-eIF4E1

    Plasmid

    #129644

    Purpose

    Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

    Depositor

    Nicholas Ingolia

    Article

    Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

    Insert

    EIF4E1 (EIF4E Human)
    

    Tags

    APEX2

    Expression

    Mammalian

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD-iPASnFR-H

    Plasmid

    #225237

    Purpose

    Bacterial expression of iPASnFR-H

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-H
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SQT1665

    Plasmid

    #78744

    Purpose

    Human expression plasmid for LbCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWPT-PDX1

    Plasmid

    #12256

    Depositor

    Didier Trono

    Article

    Ritz-Laser et al Diabetologia. 2003 Jun . 46(6):810-21.

    Insert

    Pdx1 (PDX1 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. OmpA3-PNGaseF-TEV-His6

    Plasmid

    #114274

    Purpose

    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.

    Depositor

    Martin Caffrey

    Insert

    Peptide: N-glycosidase F
    

    Tags

    OmpA3 leader sequence (for periplasmic localisati…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pR26-CMVconst

    Plasmid

    #127373

    Purpose

    Allows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Promoter

    CMV enhancer/promoter

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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