179,534 results
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Plasmid#227693PurposeGolden Gate level 1, position 2, PE2max-NC expression cassette.DepositorInsertPE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
UseCRISPRExpressionPlantPromoterCaMV 35SAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-ASAP5-Kv2.1-WPRE
Plasmid#225707PurposePan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5DepositorInsertASAP5-Kv2.1
UseAAVPromoterhuman synapsinAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV4A
Plasmid#224448PurposeRep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationRGDYNSL insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Promoter Vector
Plasmid#212939PurposeControl vector with constitutive firefly luciferase expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertluc+
UseLuciferaseMutationWTPromoterSV40Available sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
qTAG-C-dTAG-Puro
Plasmid#207727PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFH3.3_Cre-MS2x12
Plasmid#205559PurposeExpress Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)DepositorInsertCre
ExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.10_Gag-MCP-P2A-eGFP
Plasmid#205525PurposeExpress HIV Gag-MCP-P2A-eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGag-MCP
ExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
STING-miRFP680
Plasmid#200940PurposeSTING fused with miRFP680 for live cell imagingDepositorInsertsUseLentiviralAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE2_EF1a_TetOn_IRES_mCherry
Plasmid#204155PurposeLentivirus backbone expressing rtTA linked to mCherryDepositorInsertTet-On 3G
UseLentiviralExpressionMammalianPromoterEF1aAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
NbV5-eGFP
Plasmid#201473PurposeIndirect microscopy/imaging of V5-tagged proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNbV5-eGFP
TagseGFPExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1346 U6-B2M sgRNA Gag-pol v2
Plasmid#201917PurposeCas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsGag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
ExpressionMammalianPromoterCAG and Human U6Available sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-TurboID-NES_pLX304
Plasmid#202008Purposeexpresses TurboID in the mammalian cytosol, lentiviral vectorDepositorInsertFlag-TurboID-NES
UseLentiviralTagsFlagExpressionMammalianPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAWP78-PVCpnf_pvc13-Ad5Knob
Plasmid#198289PurposeProduces PVCs retargeted with knob domain from hAd5 virusDepositorInsertPVCpnf_pvc13-Ad5Knob
ExpressionBacterialAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TRUPATH Triple Gai1
Plasmid#196048PurposeEncodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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AAVS1-Bxb1-LP-TC
Plasmid#183754PurposeAAVS1 targeting construct with the Bxb1 landing pad cassette.DepositorInsertloxP-eGFP-attP-Bsd-lox257
UseCre/Lox and Synthetic BiologyExpressionMammalianAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189922PurposeAAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e
UseAAVMutationSaCas9 D10APromoterEFSAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PEmax
Plasmid#187893PurposeAll-in-one prime editor piggyBac transposon with PEmaxDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
UseCRISPR and Synthetic Biology; Piggybac transposonTagsBPSV40 NLS and c-Myc NLSExpressionMammalianPromoterCAGAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
cytoplasmic cpVenus control
Plasmid#186788PurposeIdentical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligaseDepositorInsertcytoplasmic cpVenus control
UseLentiviralExpressionMammalianAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3.1_3XFlag
Plasmid#182494PurposeEmpty plasmid containing Flag tag for C-terminal taggingDepositorInsert3xFlag with 5 Glycolic spacer
Available sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by