161,252 results

1. Integrin-β6 Headpiece I270C

   Plasmid

   #159638

   Purpose

   Contains integrin beta 6 residues 1–474 with I270C mutation

   Depositor

   Timothy Springer

   Article

   Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

   Insert

   Integrin beta 6 headpiece I270C
   

   Use

   Tags

   6x His

   Expression

   Mammalian

   Mutation

   I270C

   Promoter

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-C1 R62D actin-3XNLS P2A mCherry

   Plasmid

   #58477

   Purpose

   Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Inserts

   beta actin (ACTB Human)
   3XNLS
   P2A cleavage site
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Changed Arginine 62 to Aspartic Acid

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEmerald-Stomatin-N-16

   Plasmid

   #54262

   Purpose

   Localization: Membrane, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Stomatin (STOM Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pBAC-lacZ

   Plasmid

   #13422

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N1_hTRIM32

   Plasmid

   #69541

   Purpose

   Expresses human TRIM32 fused to c-terminal EGFP

   Depositor

   Martin Dorf

   Article

   Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960.

   Insert

   tripartite motif containing 32 (TRIM32 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDonor14-ShHELIX-KanR (CJT70)

   Plasmid

   #181793

   Purpose

   pDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-RLuc8

   Plasmid

   #87121

   Purpose

   Mammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.

   Depositor

   Sanjiv Sam Gambhir

   Article

   Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-

   Insert

   Renilla Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   A55T, C124A, S130A, K136R, A143M, M185V, M253L, a…

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOS023E: Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

   Plasmid

   #163077

   Purpose

   Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   Peredox
   

   Use

   Gateway entry vector

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. RINS1

   Plasmid

   #107290

   Purpose

   Plasmid for the expression of the RINS1 sensor

   Depositor

   Dmytro Yushchenko

   Article

   Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

   Insert

   RINS1
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC1-CMV-mito-DAAO

    Plasmid

    #141132

    Purpose

    Expresses DAAO with mitochondrial localization signal in mammalian cells

    Depositor

    Vsevolod Belousov

    Insert

    mito-DAAO
    

    Use

    Tags

    2 mitochondrial localization signals and 7xHis

    Expression

    Mammalian

    Mutation

    deleted DAAO amino acids 367-368

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmiHiFiCas9

    Plasmid

    #164832

    Purpose

    miHiFiCas9 expressing plasmid

    Depositor

    Eugene Chen

    Article

    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

    Insert

    miHiFiCas9 (contains Brex27 at sequence 5031-5138).
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mammalian

    Mutation

    change R 691 to A

    Promoter

    CMV

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-N-Flag-ASC1

    Plasmid

    #75134

    Purpose

    Expresses N-terminal Flag-tagged ASC1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    PYD and CARD domain containing (Pycard Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F23S (please see depositor comments below)

    Promoter

    CMV

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJM1-PM-RA-BlastR

    Plasmid

    #211708

    Purpose

    Lentiviral expression of a ddFP subunit (RA) fused with the lipid modification motif of lymphocyte-specific protein tyrosine kinase (Lck) for plasma membrane (PM) targetting (PM-RA)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    PM-targetted RA (Lck Synthetic)
    

    Use

    Lentiviral

    Tags

    RA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. ER-SPLICSs-cFAST10

    Plasmid

    #210431

    Purpose

    Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    ER-SPLICSs-cFAST10
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-FLT3-D835Y-V5/HIS

    Plasmid

    #236007

    Purpose

    expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    D835Y substitution

    Promoter

    CMV

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-FLT3-D835N-V5/HIS

    Plasmid

    #236008

    Purpose

    expression of the D835N kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FLT3-D835N receptor tyrosine kinase, full length (FLT3 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    D835N substitution

    Promoter

    CMV

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLminP_Luc2P_RE29

    Plasmid

    #90371

    Purpose

    SREBF1 - Sterol gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SREBPb

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-hsRPA70 (MSW#473)

    Plasmid

    #208067

    Purpose

    Expression of GFP-tagged hsRPA1 in human cells

    Depositor

    Marc Wold

    Article

    Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

    Insert

    RPA1 (RPA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAMPK alpha1 full length

    Plasmid

    #27297

    Depositor

    Morris Birnbaum

    Insert

    AMPK alpha1 (Prkaa1 Mouse)
    

    Use

    Tags

    his

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. hNedd4-2

    Plasmid

    #83433

    Purpose

    full length Nedd4L with His Xpress tag

    Depositor

    Christie Thomas

    Article

    Itani et al Am J Physiol Renal Physiol. 2005 Aug;289

    Insert

    human Nedd4L with C2 domain (NEDD4L Human)
    

    Use

    Tags

    His Tag (6x) and Xpress Epitope Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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