STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells.
Blanch-Asensio A, Grandela C, Brandao KO, de Korte T, Mei H, Ariyurek Y, Yiangou L, Mol MPH, van Meer BJ, Kloet SL, Mummery CL, Davis RP
Cell Rep Methods. 2022 Sep 22;2(10):100300. doi: 10.1016/j.crmeth.2022.100300. eCollection 2022 Oct 24. PubMed Article
Blanch-Asensio A, Grandela C, Brandao KO, de Korte T, Mei H, Ariyurek Y, Yiangou L, Mol MPH, van Meer BJ, Kloet SL, Mummery CL, Davis RP
Cell Rep Methods. 2022 Sep 22;2(10):100300. doi: 10.1016/j.crmeth.2022.100300. eCollection 2022 Oct 24. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183751 | pENTR-eGFP-attP(bxb)-*BsdR | Bxb1 landing pad cassette. |
| 183752 | pENTR-mCherry-attP(C31)-*BleoR | phiC31 landing pad cassette. |
| 183754 | AAVS1-Bxb1-LP-TC | AAVS1 targeting construct with the Bxb1 landing pad cassette. |
| 183755 | AAVS1-phiC31-LP-TC | AAVS1 targeting construct with the phiC31 landing pad cassette. |
| 183757 | Bxb1-ASAP2f | Bxb1-specific donor plasmid for intergrating a transcription unit for expression of ASAP2f voltage reporter |
| 183758 | Bxb1_jRCaMP1b | Bxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter |
| 183759 | Bxb1_miRFP703 | Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703 |
| 183760 | Bxb1_AJMA | Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703 |
| 183761 | pBR_attB(C31)_FRT | phiC31-specific donor plasmid for the cloning of payloads <20 kb |
| 183762 | pBR_attB(bxb)_lox | Bxb1-specific donor plasmid for the cloning of payloads <20 kb |
| 183763 | pBR_attB(bxb)_ccdB_lox | Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI) |
| 183764 | p15_attB(bxb)_lox | Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb) |