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STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells.

Blanch-Asensio A, Grandela C, Brandao KO, de Korte T, Mei H, Ariyurek Y, Yiangou L, Mol MPH, van Meer BJ, Kloet SL, Mummery CL, Davis RP
Cell Rep Methods. 2022 Sep 22;2(10):100300. doi: 10.1016/j.crmeth.2022.100300. eCollection 2022 Oct 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
183751pENTR-eGFP-attP(bxb)-*BsdRBxb1 landing pad cassette.
183752pENTR-mCherry-attP(C31)-*BleoRphiC31 landing pad cassette.
183754AAVS1-Bxb1-LP-TCAAVS1 targeting construct with the Bxb1 landing pad cassette.
183755AAVS1-phiC31-LP-TCAAVS1 targeting construct with the phiC31 landing pad cassette.
183757Bxb1-ASAP2fBxb1-specific donor plasmid for intergrating a transcription unit for expression of ASAP2f voltage reporter
183758Bxb1_jRCaMP1bBxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter
183759Bxb1_miRFP703Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703
183760Bxb1_AJMABxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703
183761pBR_attB(C31)_FRTphiC31-specific donor plasmid for the cloning of payloads <20 kb
183762pBR_attB(bxb)_loxBxb1-specific donor plasmid for the cloning of payloads <20 kb
183763pBR_attB(bxb)_ccdB_loxBxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)
183764p15_attB(bxb)_loxBxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

Antibodies from Article