161,202 results

1. pSG66-KcsA-Kv1.3-sfGFP

   Plasmid

   #102457

   Purpose

   Expresses a fusion protein of KcsA-Kv1.3 and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.

   Depositor

   Richard Murray

   Article

   Guo et al bioRxiv 104455

   Insert

   KcsA-Kv1.3
   

   Use

   Tags

   His tag and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   OR2-OR1-Pr

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBW2661_pCAG-PV1-iCre-N270-L1-ABI-NLS-BGHpA

   Plasmid

   #108745

   Purpose

   Expresses N-terminus to aa270 of Cre recombinase fused to ABI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa270
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   ABI,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-FRA1

   Plasmid

   #188665

   Purpose

   Expresses murine FRA1 (Fosl1)

   Depositor

   Günter Schneider

   Article

   Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

   Insert

   Fosl1 (Fosl1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-SUPREM

   Plasmid

   #205023

   Purpose

   Expressing SUPer Rna Ecogii Methyltransferase (SUPREM)

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   SUPer Rna Ecogii Methyltransferase
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-Ef1a-hMGT-PGK-puro

   Plasmid

   #172394

   Purpose

   Lentiviral expression vector for human direct cardiac reprogramming

   Depositor

   Li Qian

   Article

   Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

   Insert

   Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJYS1Ptac

   Plasmid

   #85545

   Purpose

   Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   recT
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac and unknown

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Syn-FLEX-MCS-WPRE-SV40 - GCH8

   Plasmid

   #230818

   Purpose

   AAV cloning vector for CRE-dependent expression under the Syn promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

   Plasmid

   #132405

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBOB-synP-HTB

   Plasmid

   #30195

   Depositor

   Edward Callaway, Liqun Luo

   Article

   Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

   Insert

   histone-GFP-2A-TVA receptor-2A-rabies glycoprotein
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a-LeuRS-His

    Plasmid

    #124113

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Leucyl-tRNA ligase (leuS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLifeAct_mScarlet-i_N1

    Plasmid

    #85056

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUW-tetO-RUNX3

    Plasmid

    #139812

    Purpose

    doxycycline-inducible expression of Human RUNX3 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    RUNX3 (RUNX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pF CAG luc IRES neo

    Plasmid

    #98294

    Purpose

    For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Inserts

    Firefly luciferase
    IRES
    Neomycin resistance gene (NeoR)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Mouse Ism1 with C-terminal Myc-6X-his tag

    Plasmid

    #173046

    Purpose

    Expresses Ism1 in mammalian cells

    Depositor

    Katrin Svensson

    Article

    Jiang et al Cell Metab. 2021 Jul 28. pii: S1550-4131

    Insert

    Ism1 (Ism1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-CHRNA7-YFP

    Plasmid

    #62630

    Purpose

    mammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    hAlpha7 (CHRNA7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    YFP inserted in M3-M4 loop

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. SaCas9 TadCBEd

    Plasmid

    #193845

    Purpose

    Express TadCBEd (with SaCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEd with SaCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. P2-ECFP-pA (Construct 6)

    Plasmid

    #55198

    Purpose

    Synthetic promoter responsive to gRNA2

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    mCerulean
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal Ade MLP

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTXB1-nbOcIgG-Tn5

    Plasmid

    #184285

    Purpose

    Anti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

    Depositor

    Ivan Raimondi, New York Genome Center

    Article

    Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

    Insert

    nbOcIgG-Tn5
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Talin-CS

    Plasmid

    #83377

    Purpose

    Control FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted at C-terminal o…

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG cyto-RCaMP1h

    Plasmid

    #105014

    Purpose

    cytosolic calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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