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167,150 results

Showing: 10741 - 10760 of 167150 results

  1. pSicoR p53

    Plasmid
    #12090
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF p53 shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Insert
    p53 shRNA (Trp53 Mouse)
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Mutation
    EGFP is expressed from this plasmid as a marker, …
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12090-plasmid-map-sequence-id-7226

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  4. pPFO*

    Plasmid
    #120400
    Purpose
    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.
    Depositor
    Arun Radhakrishnan
    Article
    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11
    Insert
    Mutant form of Perfringolysin O
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Amino acids 1-27 are deleted, starts at amino aci…
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120400-plasmid-map-sequence-id-233255

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  5. pDule-3-nitroTyrosine (5B)

    Plasmid
    #85498
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85498-plasmid-map-sequence-id-169862

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  7. TFORF3477

    Plasmid
    #144953
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    POU5F1 (POU5F1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144953-plasmid-map-sequence-id-391562

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  8. QQR1 variant

    Plasmid
    #138564
    Purpose
    Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    QQR1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138564-plasmid-map-sequence-id-274793

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  9. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid
    #107055
    Purpose
    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107055-plasmid-map-sequence-id-208591

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  10. TRE reporter backbone

    Plasmid
    #158678
    Purpose
    This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry
    Depositor
    Ravid Straussman
    Article
    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    mStrawberry
    Expression
    Mammalian
    Available Since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    158678-plasmid-map-sequence-id-309765

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  13. plex_307-ccdB-g418

    Plasmid
    #201141
    Purpose
    pLEX_307 plasmid with g418 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    EF-1α
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201141-plasmid-map-sequence-id-395514

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  15. DYRK1A

    Plasmid
    #38913
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DYRK1A (DYRK1A Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid
    #113865
    Purpose
    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
    Depositor
    Manfred Ogris
    Article
    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d
    Inserts
    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    113865-plasmid-map-sequence-id-220242

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  17. pBJ4

    Plasmid
    #167133
    Purpose
    A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Expression
    Bacterial
    Promoter
    Kanamycin promoter
    Available Since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167133-plasmid-map-sequence-id-330439

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Showing: 10741 - 10760 of 167150 results