A pseudovirus system enables deep mutational scanning of the full SARS-CoV-2 spike.
Dadonaite B, Crawford KHD, Radford CE, Farrell AG, Yu TC, Hannon WW, Zhou P, Andrabi R, Burton DR, Liu L, Ho DD, Chu HY, Neher RA, Bloom JD
Cell. 2023 Mar 16;186(6):1263-1278.e20. doi: 10.1016/j.cell.2023.02.001. Epub 2023 Feb 13. PubMed Article
Dadonaite B, Crawford KHD, Radford CE, Farrell AG, Yu TC, Hannon WW, Zhou P, Andrabi R, Burton DR, Liu L, Ho DD, Chu HY, Neher RA, Bloom JD
Cell. 2023 Mar 16;186(6):1263-1278.e20. doi: 10.1016/j.cell.2023.02.001. Epub 2023 Feb 13. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 204142 | HDM_omicron_B11529_IDTDNA | Expression plasmid for SARS-CoV-2 BA.1 variant spike |
| 204146 | pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR | Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes |
| 204149 | HDM_Spikedelta21_sinobiological_B.1.617.2 | Expression plasmid for SARS-CoV-2 Delta variant spike |
| 204151 | pH2rU3_ForInd_sinobiological_617.2_Spiked21_CMV_ZsGT2APurR | Lentivirus backbone with Delta spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes |
| 204152 | HDM-Hgpm2 | Lentivirus helper plasmid coding for Gag/Pol |
| 204153 | pRC-CMV-Rev1b | Lentivirus helper plasmid coding for Rev |
| 204154 | HDM-tat1b | Lentivirus helper plasmid coding for Tat |
| 204155 | pHAGE2_EF1a_TetOn_IRES_mCherry | Lentivirus backbone expressing rtTA linked to mCherry |
| 204156 | HDM_VSV_G | VSV-G expression plasmid |
| 204579 | pH2rU3_ForInd_mCherry_CMV_ZsGT2APurR | lentivirus backbone cloning vector and template for barcoded non-neutralizable standard production |