159,141 results
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PB-hEF1A-MG-U6-sgRNA
Plasmid#175505PurposeAll-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAsDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhEF1a, U6Available sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
CLYBL_Bxb1-GA_LP_Dual_TC
Plasmid#229776PurposeCLYBL targeting construct with the Bxb1-GA landing pad cassette.DepositorInsertFRT-mScarlet-attP(GA)-PuroR-F3
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpGKAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AcGFP1-ORP9-PH
Plasmid#214266PurposeMammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PHDepositorInsertAcGFP1-ORP9(1-111) (Osbpl9 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP1-10-pDEST-N
Plasmid#118368PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsHA-GFP1-10ExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-Myc-MBP-HA
Plasmid#222711PurposeExpression Myc-tagged F-box protein and MBP-HA-tagged substrate in bacterial cellsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRA108
Plasmid#209341PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mCherry fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUHD.NFATc1nuc
Plasmid#40616DepositorInsertNFATc1nuc (NFATC1 Human)
UseTagsHAExpressionMammalianMutationS172A S175A S176A S178A S179A S181A S184A S187A S…PromoterCMV2Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-Miro1 E208K/E328K
Plasmid#47894PurposeExpresses myc tagged Miro1 E208K/E328K mutantDepositorInsertMiro1 E208K/E328K (RHOT1 Human)
UseTagsmycExpressionMammalianMutationE208K/E328K, abolishes calcium bindingPromoterCMVAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CMVs.TurboRFP.WPRE.RBG
Plasmid#105548PurposeAAV expression of TurboRFP from CMV promoterDepositorHas ServiceAAV1 and AAV8InsertTurboRFP
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
enIscB-T5E
Plasmid#205411PurposeVector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoterDepositorInsertpCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
UseTagsExpressionMammalianMutationE85R+H369R+S387R+S457RPromoterCAG, hU6, CMVAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-MCS
Plasmid#84008Purposedoxycycline-inducible lentiviral vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pY3
Plasmid#50606PurposeOrigin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5DepositorInsertstyrB
tyrA
aroC
aroA
aroL
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceNov. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mAIM2-Flag
Plasmid#51537PurposeExpress Flag-tagged mAIM2 in mammalian cellsDepositorInsertAim2 (Aim2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-6XHis-human SUMO 1
Plasmid#133770Purposeexpress 6Xhis- SUMO 1 proteinDepositorInsertSUMO 1 (SUMO1 Human)
UseTags6XHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-Archon1-KGC-EGFP-ER2-WPRE
Plasmid#108423PurposeArchon1 fluorescent voltage reporter in mammalian expression plasmidDepositorInsertArchon1-KGC-EGFP-ER2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
BBa_J72214-BBa_J72090
Plasmid#40782DepositorInsertVioABCDE
UseSynthetic Biology; PhagemidTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-EGFP-PRC1
Plasmid#167540PurposeFor expression of His6-TEV-EGFP-PRC1 in bacteria.DepositorInsertPRC1 (PRC1 Human)
UseTagsEGFP and His6 tagExpressionBacterialMutationPromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-FTO-control
Plasmid#216859PurposeCIRTS RNA targeting system for targeted removal of m6A on transcript at the synapse. CIRTS-FTO-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-FTO-Calm3-U6-control gRNA
UseLentiviralTagsmCherry2ExpressionMammalianMutationPromoterSyn1, U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only
