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pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

Plasmid

#191796

Purpose

vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

Insert

humanized dCas13X

Use

CRISPR

Expression

Mammalian

Mutation

R84A, H89A, R739A, R740A, H745A, H746A, H747A

Promoter

CBh, SV40, hU6

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Hs.cargo(Cre)

Plasmid

#174863

Purpose

Expresses human Peg10 cargoRNA encoding Cre recombinase

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

Cre flanked by HsPeg10 UTRs

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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3xnls-mScarlet-I

Plasmid

#98816

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-ValRS-His

Plasmid

#124122

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Valyl-tRNA ligase (valS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag HBx

Plasmid

#42596

Depositor

Ding Xue

Article

Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

Insert

Hepatitis B virus protein X

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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SacI ML GFP Strand 11 Long

Plasmid

#164120

Purpose

Detect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

SacI ML GFP Strand 11 Long

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-Hsp70-Cas9

Plasmid

#46294

Purpose

A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

Insert

codon optimized Cas9

Use

CRISPR

Tags

3x Flag

Expression

Insect

Mutation

Silent change from C to A at bp 834 of insert

Promoter

Hsp70

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pET21a-GluRS-His

Plasmid

#124109

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutamyl-tRNA ligase (gltX Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-ABCB1_STOP

Plasmid

#221424

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

Insert

ABCB1 (ABCB1 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-NFIA

Plasmid

#141403

Purpose

Inducible Expression of NFIA

Depositor

Lorenz Studer

Article

Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

Insert

NFIA (NFIA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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GUT BuGZ FL

Plasmid

#84024

Purpose

Expression of GFP tagged BuGZ in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BuGZ (ZNF207 Human)

Use

Lentiviral

Tags

TurboGFP

Expression

Mammalian

Mutation

Q394R

Promoter

UCOE / SFFV

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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EC70188

Plasmid

#209449

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

HvU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-EXO1a

Plasmid

#111629

Purpose

Expresses human WT Exonuclease 1a (EXO1a) tagged with mCherry in mammalian cells

Depositor

Marco Muzi-Falconi

Article

Sertic et al Proc Natl Acad Sci U S A. 2011 Aug 16;10

Insert

Exonuclease 1a (EXO1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-His6dNLP

Plasmid

#105498

Purpose

Expression of N-terminally 6His tagged Drosophila nucleoplasmin-like protein

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

His6dNLP

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTubb3-MC

Plasmid

#87112

Purpose

minicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

GFP

Use

CRISPR

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 puro - CRBN (#1)

Plasmid

#166240

Purpose

sgRNA (#1) to generate a knockout of human CRBN by targeting the middle region of Exon1

Depositor

Günter Schneider

Article

Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

Insert

CRBN (CRBN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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A&C-BEmax

Plasmid

#157947

Purpose

Mammalian expression of A&C-BEmax

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

A&C-BEmax

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBBR1k-GFPuv

Plasmid

#106382

Purpose

IPTG-inducible trc promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

trc

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-irf3-shrna1

Plasmid

#127648

Purpose

Knock-down of human IRF3

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

IRF3 shRNA (IRF3 Human)

Use

Lentiviral

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/T0-GFP-PALB2

Plasmid

#71113

Purpose

mammalian expession of GFP-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV/TO

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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