166,547 results

1. pUC57BsaI

   Plasmid

   #128859

   Purpose

   Module cloning vector

   Depositor

   Gilles Charvin

   Article

   Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. RXFP4-Tango

   Plasmid

   #66495

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   RXFP4 (RXFP4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

3. PB CAG-L-3xSV40pA-L-td-sfGFP

   Plasmid

   #133574

   Purpose

   CAG tdGFP cre/lox reporter piggyBac transposon (3x SV40pA stop)

   Depositor

   Mario Capecchi

   Insert

   Tandem dimer superfolder GFP
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLENTI6.3/AR-GC-E2325

   Plasmid

   #85128

   Purpose

   exogenous AR expression

   Depositor

   Karl-Henning Kalland

   Article

   Olsen et al BMC Cancer. 2016 Jul 4;16:377. doi: 10.1

   Insert

   androgen receptor (AR Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas9 TLS1

   Plasmid

   #196977

   Purpose

   Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9 with TLS1 tag
   

   Expression

   Plant

   Promoter

   pG10-90

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-SARS-CoV-2-S-RBD-8his

   Plasmid

   #145145

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
   

   Tags

   8his tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMpGWB107

   Plasmid

   #68561

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   Citrine

   Expression

   Plant

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEP PolI

   Plasmid

   #11722

   Depositor

   Lawrence Loeb

   Article

   Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

   Insert

   DNA polymerase I EP (polA E. coli)
   

   Expression

   Bacterial

   Mutation

   D424A, I709N, A759R. Error-prone version of PolI.

   Available Since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPGKpuro

   Plasmid

   #11349

   Depositor

   Rudolf Jaenisch

   Article

   Tucker et al Genes Dev. 1996 Apr 15. 10(8):1008-20.

   Insert

   pgk-1 5' and 3' regions (Pgk1 Mouse)
   

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRGD-GmR

    Plasmid

    #74106

    Purpose

    Plasmid containing gentamicin resistance gene cassette flanked by multiple restriction sites

    Depositor

    Hans-Martin Fischer

    Article

    Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

    Insert

    aacC1
    

    Use

    Bacterial vector based on pmb1 replicon

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPR.v2.TetON_Cas13d_U6_DR

    Plasmid

    #196727

    Purpose

    Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.

    Depositor

    Bernhard Schmierer

    Insert

    RfxCas13d-T2A-miRFP670nano
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Promoter

    TRE

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbS5c-mCherry-AsLOV2*(543)

    Plasmid

    #202712

    Purpose

    Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

    Depositor

    Mary Dunlop

    Article

    Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

    Insert

    mCherry-AsLOV2*(543)
    

    Expression

    Bacterial

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lsg-GBA-1 + 2

    Plasmid

    #199276

    Purpose

    nicking sgRNA to induce or correct GBA (c. 1226 A > G) mutation using PE3

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    GBA nick
    

    Expression

    Mammalian

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-MHL: nsp10_SARS-CoV-2

    Plasmid

    #167851

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp10

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. secretedBirA-8his

    Plasmid

    #32408

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    BirA
    

    Tags

    8 His tag

    Expression

    Mammalian

    Available Since

    Jan. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2 xenopus chordin myc

    Plasmid

    #24682

    Depositor

    Edward De Robertis

    Article

    Piccolo et al Cell. 1996 Aug 23. 86(4):589-98.

    Insert

    Chordin
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pDONR221-SV2C_STOP

    Plasmid

    #161463

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SV2C (SV2C Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAcGP67A-mMD2-proA

    Plasmid

    #187878

    Purpose

    baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

    Depositor

    Bruce Beutler

    Article

    Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    MD2 (Ly96 Mouse)
    

    Expression

    Insect

    Mutation

    includes residues 19–160 of mouse MD-2

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6.2-3xFLAG-V5-ccdB

    Plasmid

    #87072

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

    Depositor

    Susan Lindquist, Mikko Taipale

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. VP64-dCas9

    Plasmid

    #177171

    Purpose

    VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

    Insert

    VP64-dCas9(D10A, H840A)
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xFLag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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