162,099 results

1. pcDNA3-RTP1S

   Plasmid

   #149364

   Purpose

   Expresses RTP1S in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

   Insert

   RTP1S (Rtp1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMC-6-GFP-P2A-NptII-9

   Plasmid

   #197741

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   EGFP-P2A-NptII bicistronic CDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXPR_501

   Plasmid

   #96922

   Purpose

   for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64

   Depositor

   David Root, John Doench

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRPA32-mCherry

   Plasmid

   #211384

   Purpose

   mammalian expression of human RPA32 C-terminally fused to mCherry

   Depositor

   Helmut Pospiech

   Insert

   RPA32 (RPA2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lvl0 mKate2 CDS

   Plasmid

   #153394

   Purpose

   Far red fluorescent protein with SV40 NLS, codon optimised for Arabidopsis thaliana

   Depositor

   Naomi Nakayama

   Article

   Andreou et al bioRxiv, March 31, 2021

   Insert

   mKate2 CDS
   

   Use

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PcDNA3.1-Flag

   Plasmid

   #210342

   Purpose

   Empty Vector for molecular cloning

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   Flag tag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. iF53 PB-Zim3-dCas9-mScarlet-Hygro

   Plasmid

   #204726

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCIneo RYR-1

   Plasmid

   #75113

   Purpose

   Clone encoding rabbit ryanodine receptor in mammalian expression vector

   Depositor

   Paul Allen, Robert Dirksen

   Article

   Nakai et al J Biol Chem. 1998 May 29;273(22):13403-6

   Insert

   Ryanodine receptor 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHMGWA-Pa14Csy4H29A

   Plasmid

   #41092

   Depositor

   Jennifer Doudna

   Article

   Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

   Insert

   Csy4
   

   Use

   Tags

   His6-MBP

   Expression

   Mutation

   His29Ala mmutation to abolish ribonuclease activi…

   Promoter

   T7

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

    Plasmid

    #120563

    Purpose

    Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR myc-MBP-SspB-iRFP-NLS

    Plasmid

    #223286

    Purpose

    An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.

    Depositor

    Jared Toettcher

    Article

    Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

    Insert

    Myc-MBP-SspB-irFP-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJ1996_v2

    Plasmid

    #140664

    Purpose

    dCas9 & Csy4

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

    Insert

    dCas9 & Csy4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

    Plasmid

    #228902

    Purpose

    For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD

    Depositor

    Bryan Roth

    Article

    Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.

    Insert

    hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)
    

    Use

    AAV and Synthetic Biology

    Tags

    HA and P2A mCitrine

    Expression

    Mammalian

    Mutation

    R108K

    Promoter

    Chicken Beta Actin

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. hSyn-somBiPOLES-mCerulean

    Plasmid

    #154945

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

    Plasmid

    #141378

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. Klc1-GFP

    Plasmid

    #186617

    Purpose

    Encodes Kinesin Light Chain 1 fused to GFP

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Kinesin Light Chain 1 (KLC1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-TRE-fDIO-GFP-IRES-tTA

    Plasmid

    #118026

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-fDIO-GFP-IRES-tTA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See depositor comments below

    Promoter

    TRE

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTet-O-ATOH1-T2A-PuroR

    Plasmid

    #162342

    Purpose

    Lentiviral expression of ATOH1 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    ATOH1 (ATOH1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR

    Plasmid

    #192636

    Purpose

    Expresses ERT2-CreN59-nMag-NLS with mCherry marker.

    Depositor

    Yingxiao Wang

    Article

    Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

    Insert

    ERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

    Plasmid

    #226105

    Purpose

    ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    atf4 (ATF4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains uORF1-2 of ATF4, whose stability can ser…

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge