159,157 results

1. CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1

   Plasmid

   #138418

   Purpose

   CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1

   Depositor

   Jian Xu

   Article

   Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   BioTAP-tag

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-hAtg13

   Plasmid

   #22875

   Depositor

   Noboru Mizushima

   Article

   Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

   Insert

   autophagy-related genes 13 (ATG13 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pOpen-Eco31I

   Plasmid

   #165506

   Purpose

   Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   Eco31I
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pICSL01009::AtU6p

   Plasmid

   #46968

   Purpose

   Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851)

   Plasmid

   #164450

   Purpose

   AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Has Service

   AAV PHP.eB

   Insert

   iCre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBetaGlobin

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJS167

   Plasmid

   #48881

   Purpose

   Positive feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

   Inserts

   araC gene with ssrA tag
   monomeric yeast-enhanced green fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   ssrA (TSAANDENYALAA)

   Expression

   Bacterial

   Mutation

   Promoter

   Para/lac

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJS169

   Plasmid

   #48882

   Purpose

   Negative feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

   Insert

   lacI with ssrA tag
   

   Use

   Synthetic Biology

   Tags

   ssrA (TSAANDENYALAA)

   Expression

   Bacterial

   Mutation

   Promoter

   Para/lac

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR myc-MBP-SspB-iRFP-NLS

   Plasmid

   #223286

   Purpose

   An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.

   Depositor

   Jared Toettcher

   Article

   Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

   Insert

   Myc-MBP-SspB-irFP-NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLNHA-C1-HsXRN1

   Plasmid

   #66596

   Purpose

   expresses lambdaN-HA-tagged XRN1 in mammalian cells

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   XRN1 (XRN1 Human)
   

   Use

   Tags

   lambdaN-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA6-N-Flag-SREBP1

    Plasmid

    #134283

    Purpose

    Expresses Flag-tagged SREBP1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP1 isoform a precursor

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA-3xFLAG-Pontin

    Plasmid

    #51635

    Purpose

    Mammalian expression of 3xFLAG tagged pontin, N-terminal.

    Depositor

    Steven Artandi

    Article

    Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

    Insert

    Pontin (RUVBL1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 HA-YY1

    Plasmid

    #104395

    Purpose

    HA-YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    YY1 (YY1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. ER-HA-mNG21-10

    Plasmid

    #141165

    Purpose

    Split Fluorescent Reporter for ER Retrograde Trafficking

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    ER-HA-mNG21-10-KDEL
    

    Use

    Lentiviral

    Tags

    Signal Sequence, KDEL and HA

    Expression

    Mammalian

    Mutation

    K128M, S142T, R150M, G172V and K213M

    Promoter

    Ef1a

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLARRY-EGFP

    Plasmid

    #140025

    Purpose

    Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

    Depositor

    Fernando Camargo

    Article

    Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBiT3.1-C_FBXW7:1-707

    Plasmid

    #211104

    Purpose

    Mammalian expression of full-length human FBXW7. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    FBXW7:M1-K707 (FBXW7 Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV pCAG-FLEX-EGFP-WPRE

    Plasmid

    #51502

    Purpose

    Can be used to generate AAV virus that will express EGFP in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1a- gfp-2a-puro

    Plasmid

    #129443

    Purpose

    expression of GFP and Puro R

    Depositor

    Oskar Laur

    Insert

    gfp-puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

    Plasmid

    #87803

    Purpose

    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter, tetracycline operator

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a/Cas9-Cys

    Plasmid

    #53261

    Purpose

    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine

    Depositor

    Hyongbum Kim

    Article

    Ramakrishna et al Genome Res. 2014 Apr 2.

    Insert

    Cas9-Cys
    

    Use

    Tags

    6X His tag, HA epitope, and NLS

    Expression

    Bacterial

    Mutation

    Additional Cysteine is added to the C-terminal re…

    Promoter

    T7 Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. NES-mCherry-cPHx3

    Plasmid

    #116862

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    amino acids 169-329 (tandem trimer)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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