173,763 results

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p2T-CAG-CIBN-eGFP-CAAX-Puro

Plasmid

#240804

Purpose

Constitutive expression of CIBN-eGFP

Depositor

Hans Clevers

Article

Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

Insert

CIBN-eGFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(ACT4)

Plasmid

#235060

Purpose

Expresses Tad-CBE(ACT4) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACT4)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-mRuby3

Plasmid

#74252

Purpose

Mammalian expression of tandem fusion of mClover3-mRuby3

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

mClover3-mRuby3

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pDD282

Plasmid

#66823

Purpose

GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

GFP-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized GFP

Expression

Worm

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLXI_TRC401 SMARCB1 var 2

Plasmid

#111182

Purpose

Inducible vector (untagged) with SMARCB1 variant 2

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

BAF47 (SMARCB1 Human)

Use

Lentiviral

Promoter

TRE

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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OMM-Flag-APEX2-SOPP3

Plasmid

#229073

Purpose

Expresses APEX2-SOPP3 targeted to outer mitochondrial membrane

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in outer mitochondrial membrane (APEX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-mem-seTurboID

Plasmid

#237887

Purpose

AAV vector for Cre-dependent expression of membrane-tethered seTurboID

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

mem-seTurboID

Use

AAV and Cre/Lox

Tags

FLAG tag and GAP43 palmitoylation signal

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_Z17-ehaA_LacI

Plasmid

#186313

Purpose

Plasmid for inducible E coli expression of the Z17 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

Z17-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis).iGluSnFR

Plasmid

#41732

Purpose

Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Insert

GltI based Glutamate Biosensor

Use

Glutamate biosensor

Tags

Myc, PDGFR transmembrane helix (aa 513-561), and …

Expression

Mammalian

Mutation

cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

Promoter

CMV

Available Since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pLX317-hcRED

Plasmid

#115440

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

hcRED

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-2P

Plasmid

#179458

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-FLAG-NEK9

Plasmid

#168269

Purpose

Expresses NEK9 in mammalian cells

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

NEK9 (NEK9 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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PR_GEF (2XPDZ-mCherry-Larg (DH)

Plasmid

#80407

Purpose

Recruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)

Tags

mCherry

Expression

Mammalian

Mutation

DH domain only (aa 766-997)

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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GLASTp-DsRed2

Plasmid

#17706

Depositor

Nicholas Gaiano

Article

Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

Insert

GLASTp (SLC1A3 Human)

Tags

DsRed2

Expression

Mammalian

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

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GFP-Rab35 WT

Plasmid

#47424

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 (RAB35 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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IFNGR1-SmBiT Vector

Plasmid

#238819

Purpose

Express IFNGR1-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

IFNGR1 (IFNGR1 Human)

Tags

SmBiT

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pB-tre-dCas9-VPR

Plasmid

#227506

Purpose

piggyBac integration and dox-inducible expression of VPR-dCas9

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

VPR-dCas9

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12558 - pAAV-AiE0390m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2558)

Plasmid

#208141

Purpose

AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-MITF-A

Plasmid

#38132

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

MITF-A (MITF Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3 Gag-imCherry

Plasmid

#208882

Purpose

codes for Gag from HIV-1 with an internal mCherry fluorescent protein

Depositor

Raphael Gaudin

Article

Diana Brychka et al bioRxiv

Insert

HIV-1 Gag

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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