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Substoichiometric Hydroxynonenylation of a Single Protein Recapitulates Whole-Cell-Stimulated Antioxidant Response.
Parvez S, Fu Y, Li J, Long MJ, Lin H, Lee DK, Hu GS, Aye Y
J Am Chem Soc. 2014 Dec 31.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
62454pet28a-His6-Keap1His6 tagged Keap1 for E.coli expression
62455pet28a-His6-Halo-TEV-Nrf2Halo-His6 tagged Nrf2 for E.coli expression
62456pet28a-His6-Halo-Tev-Keap1His6-halo tagged human Keap1 protein for E.coli expression
62457pMIR-Dsred-IRES-His Keapexpresses his tagged human Keap1 protein
62458pMIR-Dsred-IRES-His-Halo-Tev-Keap C151Sexpresses his-tagged C151S human Keap1 protein
62459pMIR-Dsred-IRES-His-Halo-Tev-Keap C288Sexpresses his-tagged C288S human Keap1 protein
62460pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288Sexpresses C151S and C288S double mutant human Keap1 protein

Antibodies from Article