161,982 results

1. pcDNA3.1-Beta2

   Plasmid

   #166772

   Purpose

   Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GNB2 (GNB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFA6a-13Myc-His3MX6

   Plasmid

   #41602

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   13Myc, A. gossypii translation elongation factor …

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115216

   Purpose

   Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   Pin2Cas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBABE puro H-Ras V12

   Plasmid

   #9051

   Purpose

   Retroviral expression of a constitutively active form of H-Ras

   Depositor

   William Hahn

   Insert

   Hras V12 (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   G12V, constitutively active

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. BRAF_pLX307

   Plasmid

   #98322

   Purpose

   Lentiviral expression of BRAF

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   BRAF (BRAF Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)

   Plasmid

   #114067

   Purpose

   T7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guides

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   entry vector for AsCas12a crRNAs
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. IRF8-2A-neo

   Plasmid

   #190621

   Purpose

   Lentiviral expression plasmid of human IRF8, neomycin selection

   Depositor

   Junwei Shi

   Article

   Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

   Insert

   IRF8 (IRF8 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. plenti-UbcP-HA-CRBN-pGK-HYG

   Plasmid

   #107378

   Purpose

   Lentiviral expression of HA-CRBN

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   UbcP

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV phSyn1(S)-FLEX-EGFP-WPRE

   Plasmid

   #51504

   Purpose

   Can be used to generate AAV virus that will express EGFP in the presence of Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   phSyn1

   Available since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPP441

    Plasmid

    #158801

    Purpose

    Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-2
    

    Use

    Tags

    2xSV40NLS-2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-Lamin-A

    Plasmid

    #206027

    Purpose

    Expresses GFP-tagged Lamin A in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Lamin-A (LMNA Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLD3 translation factors

    Plasmid

    #117762

    Purpose

    BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

    Depositor

    Anthony Forster

    Article

    Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Translation factor cistrons 22-30
    

    Use

    Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLD2 translation factors

    Plasmid

    #117761

    Purpose

    BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

    Depositor

    Anthony Forster

    Article

    Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Translation factor cistrons 14-21
    

    Use

    Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-DIO-Ef1a-TVA-2A-N2cG

    Plasmid

    #172360

    Purpose

    Targeting envA-pseodotyped RVdG vectors for specific retrograde labeling specifically from cre-expressing neurons

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-N2cG
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LZ10 PBREBAC-H2BHalo

    Plasmid

    #91564

    Purpose

    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.

    Depositor

    James Zhe Liu

    Article

    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1

    Insert

    histone H2B-HaloTag
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. tol2-mpeg1-mcherry

    Plasmid

    #58935

    Purpose

    labels macrophages with mcherry

    Depositor

    Anna Huttenlocher

    Insert

    mpeg1-mcherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    mpeg1

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

    Plasmid

    #141386

    Purpose

    Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-M (M SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pRK5-HA-metap2

    Plasmid

    #100512

    Purpose

    Expresses HA tagged metap2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    Metap2 (METAP2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJC53.2

    Plasmid

    #26536

    Purpose

    T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

    Depositor

    Phillip Newmark

    Article

    Collins et al PLoS Biol. 2010 . 8(10):e1000509.

    Type

    Empty backbone

    Use

    T/a cloning vector for dsrna generation and the g…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115208

    Purpose

    Expresses PinCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PinCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge