166,137 results

1. pTCF236 HLA-B*40:01

   Plasmid

   #180463

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   HLA-B*40:01
   

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Available Since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Anti-6xHis [N144/14-rat IgG2a] - Chimeric

   Plasmid

   #227037

   Purpose

   Mammalian expression plasmid of anti-6xHis rat IgG2a R-mAb. Derived from hybridoma N144/14.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-6xHis recombinant mouse monoclonal antibody.
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEV53D

   Plasmid

   #44168

   Depositor

   John Olsen

   Article

   Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

   Insert

   Equine infectious anemia virus (EIAV) gag-pol,rev, and S2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   A131T mutation in gag; Deletion of viral LTR sequ…

   Promoter

   CMV

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINDUCER20-α-Syn

   Plasmid

   #92200

   Purpose

   Retroviral overexpression vector (doxycycline-inducible) for α-Syn

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   SNCA (SNCA Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-TLR3-CFP

   Plasmid

   #13641

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 3 (TLR3 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Available Since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. Synapsin-cyto-iATPSnFR1.0

   Plasmid

   #102556

   Purpose

   expresses cytoplasmic ATP sensor in neurons

   Depositor

   Baljit Khakh

   Article

   Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

   Insert

   iATPSnFR1.0
   

   Use

   AAV

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGTM_COV2_NSP5_004_SUMO

   Plasmid

   #190062

   Purpose

   Expresses SARS-CoV-2 3CLpro for the expression of the active mature protease

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   3CLpro (ORF1ab SARS-CoV-2)
   

   Tags

   6X-His-Sumo

   Expression

   Bacterial

   Promoter

   T7 lac

   Available Since

   June 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pDUAL SRBI (GFP)

   Plasmid

   #86979

   Purpose

   Lentiviral expression construct encoding SR-B1 and GFP from separate promoters

   Depositor

   Joe Grove

   Insert

   SCARB1 (SCARB1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV/hPGK

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSADdeltaG-DsRed2

   Plasmid

   #32638

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Insert

   DsRed2
   

   Use

   Rabies virus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. p(HA)HIF1alpha (P402A,P564A)

    Plasmid

    #52636

    Purpose

    mammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutation

    Depositor

    Eric Huang

    Article

    Kageyama et al FASEB J. 2004 Jun;18(9):1028-30. Epub 20

    Insert

    HIF1alpha P402A, P564A (HIF1A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    P402A, P564A

    Promoter

    CMV

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLIB-GST-3C-CDK1

    Plasmid

    #177011

    Purpose

    Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CDK1 (CDK1 Human)
    

    Tags

    GST-3C

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL1777 (pEffector)

    Plasmid

    #160731

    Purpose

    Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins and crRNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. FABP1A

    Plasmid

    #42344

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FABP1A (FABP1 Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-CAG-miniGFP1-P2A-FusionRed

    Plasmid

    #188732

    Purpose

    Express miniGFP1 and FusionRed in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP1
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. WT USP7 catalytic

    Plasmid

    #227201

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. ratGlp1-R

    Plasmid

    #14944

    Depositor

    Bernard Thorens

    Article

    Thorens et al Proc Natl Acad Sci U S A. 1992 Sep 15. 8

    Insert

    Glp1-R (Glp1r Rat)
    

    Expression

    Mammalian

    Available Since

    June 7, 2007

    Availability

    Academic Institutions and Nonprofits only

17. myrAkt delta4-129

    Plasmid

    #10841

    Depositor

    Richard Roth

    Article

    Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

    Insert

    Akt (AKT1 Human)
    

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    Deletion of PH domain (aa4-129)

    Promoter

    SV40

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCre-P2A-mCherry

    Plasmid

    #212105

    Purpose

    Expression of Cre and mCherry in mammalian cells

    Depositor

    Daekee Lee

    Insert

    Cre recombinase and mCherry
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. ampl43

    Plasmid

    #161830

    Purpose

    pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

    Depositor

    Saeed Tavazoie

    Article

    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-Ef1a-hMGT-PGK-puro

    Plasmid

    #172394

    Purpose

    Lentiviral expression vector for human direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

    Insert

    Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
    

    Use

    Lentiviral

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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