159,238 results

1. SpdCas9 KRAB

   Plasmid

   #188499

   Purpose

   Expresses FLAG tagged SpdCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-dTAG-Zeo

   Plasmid

   #207738

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAAV-hSyn-ConVERGD-(HA)hM4Di-W3SL

   Plasmid

   #218759

   Purpose

   Provides AND intersectional (Cre+Flp) expression of HA-hM4Di

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   hM4Di
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJA54 HLA-A*03:01

   Plasmid

   #180451

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   HLA-A*03:01
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Promoter

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMH-EF1-GFP

   Plasmid

   #133018

   Purpose

   (Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under EF1 promoter.

   Depositor

   Michael Huen

   Article

   Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

   Type

   Empty backbone

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Dec. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N1-GRASP55

   Plasmid

   #137708

   Purpose

   Eukaryotic expression of rat GRASP55 full-length

   Depositor

   Yanzhuang Wang

   Insert

   GRASP55
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUAS-mCherry-NLS

   Plasmid

   #87695

   Purpose

   Gal4VP16 driven expression of mCherry-NLS in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   mCherry-NLS
   

   Use

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-UtrCH

   Plasmid

   #26740

   Depositor

   William Bement

   Article

   Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

   Insert

   Utrophin
   

   Use

   Reporter for f-actin

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Calponin homology domain of utrophin (aa# 1-261)

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3xFLAG-CMV14-GINIP

   Plasmid

   #204845

   Purpose

   Express 3x FLAG tagged-GINIP in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

   Insert

   PHF24 (KIAA1045 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Pur-mEGFP

    Plasmid

    #186329

    Purpose

    Fluorescent reporter system

    Depositor

    Bertrand Collet

    Insert

    mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Murine E-cadherin mCherry

    Plasmid

    #71366

    Purpose

    mammalian expression of murine E-caderin with mCherry fusion

    Depositor

    Alpha Yap

    Article

    Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

    Insert

    E-cadherin (Cdh1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti CMVtight eGFP Hygro (w783-1)

    Plasmid

    #26585

    Purpose

    2nd generation lentiviral vector

    Depositor

    Eric Campeau

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Lentiviral; Tet-on advanced vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-US-ECasE

    Plasmid

    #83961

    Purpose

    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.

    Depositor

    Eleanor Chen

    Insert

    ERT2-spCas9-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    please see depositor comments below

    Promoter

    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCis GFPuv-SS Himar1 A7

    Plasmid

    #84686

    Purpose

    Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

    Depositor

    Ulrike Munderloh

    Article

    Munderloh et al Intracellular Pathogens II: Rickettsiale

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5-mEGFP-DVL1-WT

    Plasmid

    #194586

    Purpose

    Mammalian Expression of mEGFP-DVL1 WT Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    DVL1 (DVL1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. VRK1-dTag-puro

    Plasmid

    #199651

    Purpose

    Expresses VRK1 C-term. Tagged with dTAG

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    dTAG

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX307 ASC

    Plasmid

    #163977

    Purpose

    Mammalian expression vector encoding ASC (for example, to assess ASC-speck formation or inflammasome signalling assays)

    Depositor

    Daniel Bachovchin

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    ASC (PYCARD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330S-7

    Plasmid

    #58783

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG/ATP11A-HA

    Plasmid

    #209218

    Purpose

    Mammalian expression of ATP11A

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP11A (ATP11A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

    Plasmid

    #67982

    Purpose

    Cas9 activity reporter with mCherry and GFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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