161,916 results

1. pGGDestTol2LC-2sgRNA

   Plasmid

   #64240

   Purpose

   destination vector for two U6x:sgRNA cassettes

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-H-Ras_V12

   Plasmid

   #39504

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

   Insert

   HRAS V12 (HRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV5-ActRIIB

   Plasmid

   #13501

   Depositor

   Jeff Wrana

   Article

   Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

   Insert

   Activin receptor IIB (Acvr2b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pHSN401

   Plasmid

   #50588

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   3×FLAG-NLS-zCas9-NLS
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   maize codon optimized

   Promoter

   2×35Sp and AtU6-26p

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. AB-GFP

   Plasmid

   #72203

   Purpose

   screen AB42 variants for aggregation

   Depositor

   Michael Hecht

   Article

   Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

   Insert

   AB42 (APP Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-TetOminiCMV-OSKM

   Plasmid

   #136541

   Purpose

   Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Sox2, Pou5f1, Myc, Klf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_Lifeact-mCherry

   Plasmid

   #67302

   Purpose

   Fluorescent indicator for F-actin

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

   Insert

   Lifeact-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PGK-DTAG-CTERM-GFP

   Plasmid

   #185770

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, dTAG

   Expression

   Mutation

   none

   Promoter

   PGK

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA GFP10-ZIP

   Plasmid

   #182241

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP10

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   GFP10-ZIP
   

   Use

   Tags

   split-GFP10 tripartite

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAS9counter

    Plasmid

    #107192

    Purpose

    Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

    Depositor

    Steve Salipante

    Article

    Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

    Inserts

    Cas9
    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    prab17

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. DsRed2-C1

    Plasmid

    #54611

    Purpose

    Localization: C1 Cloning Vector, Excitation: 558, Emission: 583

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET-28a (+)-hTBP

    Plasmid

    #171073

    Purpose

    Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    TATA-box-binding protein (TBP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. VPS29-YFP

    Plasmid

    #174520

    Purpose

    Expresses human VPS29 with a C-terminal YFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Rojas et al J Cell Biol. 2008 Nov 3;183(3):513-26. d

    Insert

    VPS29 (VPS29 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. beta2-spectrin-HA

    Plasmid

    #31070

    Depositor

    Vann Bennett

    Insert

    beta2-spectrin (SPTBN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti_CMV_PsCatCh2.0-YFP

    Plasmid

    #186978

    Purpose

    Expresses PsCatCh2.0 channelrhodopsin in mammalian cells

    Depositor

    Adam Cohen

    Article

    Hayward et al eLife. 2023 Oct 6;12:RP86833. doi: 10.75

    Insert

    PsCatCh2.0-YFP
    

    Use

    Lentiviral

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Beta4

    Plasmid

    #166773

    Purpose

    Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GNB4 (GNB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP

    Plasmid

    #40640

    Depositor

    Brenda Lilly

    Article

    Zhao et al J Biol Chem. 2012 May 11;287(20):16111-2

    Insert

    NICD3 (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG_GPHN.FingR-eGFP-CCR5TC

    Plasmid

    #46296

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    GPHN.FingR-eGFP-CCR5TC (GPHN Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Ecoli PancACe

    Plasmid

    #215712

    Purpose

    Expresses acetyl-CoA biosensor in Ecoli

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Reduced Expression GFP beta actin

    Plasmid

    #31502

    Depositor

    Tim Mitchison, Rick Horwitz

    Article

    Watanabe et al Science. 2002 Feb 8. 295(5557):1083-6.

    Insert

    beta actin (ACTB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    91-544 of enhancer region of CMV promoter deleted

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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