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Addgene

pMM2-hPMCA1b
(Plasmid #47758)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47758 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMM2
  • Backbone manufacturer
    Adamo, H.P. et al. (1992) Biochem. J. 285, 791-797
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PMCA1
  • Alt name
    ATP2B1
  • Alt name
    ATPase, Ca++ transporting, plasma membrane 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3662
  • Entrez Gene
    ATP2B1 (a.k.a. PMCA1, PMCA1kb)
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer pMT2-F
  • 3′ sequencing primer pMT2-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that the PMCA sequence contains several point mutations, two of which lead to a change in amino acids (Val->Ile, Lys->Arg).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMM2-hPMCA1b was a gift from John Penniston & Emanuel Strehler (Addgene plasmid # 47758 ; http://n2t.net/addgene:47758 ; RRID:Addgene_47758)
  • For your References section:

    Overexpression of the erythrocyte plasma membrane Ca2+ pump in COS-1 cells. Adamo HP, Verma AK, Sanders MA, Heim R, Salisbury JL, Wieben ED, Penniston JT. Biochem J. 1992 Aug 1;285 ( Pt 3):791-7. PubMed 1323273