pMM2-hPMCA1b
(Plasmid #47758)

• Purpose: mammalian expression of human PMCA1b
• Depositing Labs: John Penniston, Emanuel Strehler
• Publication: Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

Backbone

• Vector backbone: pMM2
• Backbone manufacturer: Adamo, H.P. et al. (1992) Biochem. J. 285, 791-797
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PMCA1
• Alt name: ATP2B1
• Alt name: ATPase, Ca++ transporting, plasma membrane 1
• Species: H. sapiens (human)
• Insert Size (bp): 3662
• Entrez Gene: ATP2B1 (a.k.a. PMCA1, PMCA1kb)
• Promoter: SV40

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: pMT2-F
• 3′ sequencing primer: pMT2-R

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that the PMCA sequence contains several point mutations, two of which lead to a change in amino acids (Val->Ile, Lys->Arg).