pMM2-rPMCA2a
(Plasmid #47762)

• Purpose: mammalian expression of rat PMCA2a
• Depositing Labs: John Penniston, Emanuel Strehler
• Publication: Elwess et al J Biol Chem. 1997 Jul 18;272(29):17981-6.

Backbone

• Vector backbone: pMM2
• Backbone manufacturer: Adamo, H.P. et al. (1992) Biochem. J. 285, 791-797
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PMCA2a
• Alt name: ATP2B2
• Alt name: ATPase, Ca++ transporting, plasma membrane 2
• Species: R. norvegicus (rat)
• Entrez Gene: Atp2b2 (a.k.a. PMCA2)
• Promoter: SV40

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: pMT2-F
• 3′ sequencing primer: pMT2-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry