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(Plasmid #47589)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47589 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    ATPase, Ca++ transporting, plasma membrane 4
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    K442R and P673L relative to NP_001675.3
  • Entrez Gene
    ATP2B4 (a.k.a. ATP2B2, MXRA1, PMCA4, PMCA4b, PMCA4x)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HPMCA4x/b coding sequence starts with amino acid 2 (Thr) at pos 1354.

This plasmid carries two missense mutations that cause K442R and P663L in the amino acid sequence of hPMCA4b. The depositing lab did not notice any functional consequences in the assays they performed. However, these were mostly concerned with membrane localization and did not include any enzymatic functional studies. Thus, it is not know whether (and how) these two mutations affect the calcium-pumping enzymatic function of the encoded GFP-hPMCA4b protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-hPMCA4b was a gift from Emanuel Strehler (Addgene plasmid # 47589 ; ; RRID:Addgene_47589)
  • For your References section:

    Alternative splicing of the first intracellular loop of plasma membrane Ca2+-ATPase isoform 2 alters its membrane targeting. Chicka MC, Strehler EE. J Biol Chem. 2003 May 16;278(20):18464-70. Epub 2003 Mar 6. 10.1074/jbc.M301482200 PubMed 12624087