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CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den Heuvel S, Boxem M
Genetics. 2013 Aug 26.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
47941pMB60Can be used to generate synthetic guide in vitro from T7 promotor
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47943pMB70Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences
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47944pMB62Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter
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47945pMB63Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter
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47946pMB66Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter
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47947pMB67Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter
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