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Addgene

pMB60
(Plasmid #47941)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47941 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMK
  • Backbone manufacturer
    Geneart
  • Total vector size (bp) 2286
  • Vector type
    Worm Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synthetic guide RNA
  • Alt name
    sgRNA
  • Species
    Synthetic
  • Insert Size (bp)
    80

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information on Boxem Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/boxem/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMB60 was a gift from Mike Boxem (Addgene plasmid # 47941 ; http://n2t.net/addgene:47941 ; RRID:Addgene_47941)
  • For your References section:

    CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans. Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den Heuvel S, Boxem M. Genetics. 2013 Aug 26. 10.1534/genetics.113.156299 PubMed 23979586