164,223 results

1. pDN77

   Plasmid

   #61347

   Purpose

   mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

   Depositor

   Roland Eils, Barbara Di Ventura

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   GS linker-AsLov2-biNLS2 and NES IkBa

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDawn-Ag43

   Plasmid

   #107742

   Purpose

   Expresses Ag43 under the control of a blue-light transcriptional regulator

   Depositor

   Ingmar Riedel-Kruse

   Article

   Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

   Insert

   Ag43 (flu )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   removed all pstI cut sites with silent mutations,…

   Promoter

   pLambda

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH6HTC_PRMT1-Halo

   Plasmid

   #175334

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   PRMT1 (PRMT1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHPRT-DRGFP

   Plasmid

   #26476

   Purpose

   DR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting sequences

   Depositor

   Maria Jasin

   Article

   Pierce et al Genes Dev. 2001 Dec 15. 15(24):3237-42.

   Insert

   pHPRT-DRGFP
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. mscv_EGFP-NUP98-KDM5A

   Plasmid

   #238293

   Purpose

   For overexpression of EGFP-NUP98-KDM5A

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   EGFP-NUP98-KDM5A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSynapsin-Flpo

   Plasmid

   #60663

   Purpose

   Expresses pAAV-hSynapsin-Flpo

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   Flpo
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSynapsin1

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. OPEN HLA-A*02:01-BirA

   Plasmid

   #215569

   Purpose

   E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   BirA

   Expression

   Bacterial

   Mutation

   Glycine 120 to Cysteine

   Promoter

   T7

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_SEPT2-msfGFP

   Plasmid

   #180318

   Purpose

   mammalian expression of human SEPT2 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN2 (SEPTIN2 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PCDNA3-muOR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

   Plasmid

   #113611

   Purpose

   PPI reporter

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

   Insert

   mu opioid receptor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBW-m4134902

    Plasmid

    #151912

    Purpose

    Mammalian expression plasmid for Human coronavirus 229E nucleocapsid

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    Human coronavirus 229E nucleocapsid protein (N Human coronavirus 229E)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pFB2E

    Plasmid

    #195288

    Purpose

    Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK EGFP 2A Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-ccdB-3xFLAG

    Plasmid

    #214984

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-iRFP670-CaaX

    Plasmid

    #170464

    Purpose

    Expresses iRFP670 fluorophore targeted to the plasma membrane using a prenylation motif

    Depositor

    John James

    Article

    Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

    Insert

    iRFP670-CaaX
    

    Use

    Lentiviral

    Tags

    CaaX

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCit(S1-S3)3-SAH

    Plasmid

    #228067

    Purpose

    Mammalian expression of coiled coil segments (S1-S3)x3, with long rigid linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    mCitrine, S1, S3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. TagBFP(S2)3(S4)3-gs

    Plasmid

    #228068

    Purpose

    Mammalian expression of coiled coil segments (S2)x3-(S4)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    BFP, S2, S4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

    Plasmid

    #117656

    Purpose

    Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Inserts

    sfCherry2(1-10)
    sfCherry2(11)-SpyCatcher002
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q60

    Plasmid

    #111749

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q60 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR1a-tagRFP-KDEL

    Plasmid

    #114177

    Purpose

    Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    tagRFP-KDEL
    

    Use

    Gateway entry clone

    Tags

    KDEL retention signal and signal peptide

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-CjCas9

    Plasmid

    #89754

    Purpose

    Expression of CjCas9 with His tag in E.coli

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    6XHis, HA, and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. ODD-Luciferase-pcDNA3

    Plasmid

    #18965

    Depositor

    William Kaelin

    Article

    Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

    Insert

    ODD-Firefly Luciferase (HIF1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HIF1a ODD sequence fused in frame to luciferase O…

    Promoter

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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