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Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression.
Pierson Smela MD, Kramme CC, Fortuna PRJ, Adams JL, Su R, Dong E, Kobayashi M, Brixi G, Kavirayuni VS, Tysinger E, Kohman RE, Shioda T, Chatterjee P, Church GM
Elife. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
192892pHDR-FOXL2-T2A-tdTomato-PuroTKHDR donor for inserting FOXL2-T2A-tdTomato reporter
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192893pX330-FOXL2-ctermExpresses Cas9 and sgRNA targeting the C-terminus region of FOXL2
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192894pPB-cT3G-cERP2-TCF21Barcoded piggybac transposon vector with Dox-inducible expression of TCF21
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192895pPB-cT3G-cERP2-JUNBarcoded piggybac transposon vector with Dox-inducible expression of JUN
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192896pPB-cT3G-cERP2-GATA4Barcoded piggybac transposon vector with Dox-inducible expression of GATA4
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192897pPB-cT3G-cERP2-CEBPDBarcoded piggybac transposon vector with Dox-inducible expression of CEBPD
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192898pPB-cT3G-cERP2-EMX2Barcoded piggybac transposon vector with Dox-inducible expression of EMX2
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192899pPB-cT3G-cERP2-MYCBarcoded piggybac transposon vector with Dox-inducible expression of MYC
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192900pPB-cT3G-cERP2-NR2F2Barcoded piggybac transposon vector with Dox-inducible expression of NR2F2
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192901pPB-cT3G-cERP2-FOSBBarcoded piggybac transposon vector with Dox-inducible expression of FOSB
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192903pPB-cT3G-cERP2-ZBTB16Barcoded piggybac transposon vector with Dox-inducible expression of ZBTB16
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192904pPB-cT3G-cERP2-YBX1Barcoded piggybac transposon vector with Dox-inducible expression of YBX1
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192905pPB-cT3G-cERP2-LHX1Barcoded piggybac transposon vector with Dox-inducible expression of LHX1
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192906pPB-cT3G-cERP2-WT1Barcoded piggybac transposon vector with Dox-inducible expression of WT1
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192907pPB-cT3G-cERP2-JUNBBarcoded piggybac transposon vector with Dox-inducible expression of JUNB
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192908pPB-cT3G-cERP2-KLF4Barcoded piggybac transposon vector with Dox-inducible expression of KLF4
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192909pPB-cT3G-cERP2-KLF6Barcoded piggybac transposon vector with Dox-inducible expression of KLF6
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192910pPB-cT3G-cERP2-HOXC9Barcoded piggybac transposon vector with Dox-inducible expression of HOXC9
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192911pPB-cT3G-cERP2-ATF4Barcoded piggybac transposon vector with Dox-inducible expression of ATF4
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192912pPB-cT3G-cERP2-PPARGBarcoded piggybac transposon vector with Dox-inducible expression of PPARG
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192913pPB-cT3G-cERP2-EGR1Barcoded piggybac transposon vector with Dox-inducible expression of EGR1
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192914pPB-cT3G-cERP2-FOXL2Barcoded piggybac transposon vector with Dox-inducible expression of FOXL2
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192915pPB-cT3G-cERP2-NR5A1Barcoded piggybac transposon vector with Dox-inducible expression of NR5A1
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192916pPB-cT3G-cERP2-TSC22D3Barcoded piggybac transposon vector with Dox-inducible expression of TSC22D3
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192917pPB-cT3G-cERP2-MAFFBarcoded piggybac transposon vector with Dox-inducible expression of MAFF
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192918pPB-cT3G-cERP2-ELK1Barcoded piggybac transposon vector with Dox-inducible expression of ELK1
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192919pPB-cT3G-cERP2-NR1H2Barcoded piggybac transposon vector with Dox-inducible expression of NR1H2
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192920pPB-cT3G-cERP2-TOX2Barcoded piggybac transposon vector with Dox-inducible expression of TOX2
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192921pPB-cT3G-cERP2-LHX9Barcoded piggybac transposon vector with Dox-inducible expression of LHX9
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192922pPB-cT3G-cERP2-ZFPM2Barcoded piggybac transposon vector with Dox-inducible expression of ZFPM2
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192923pPB-cT3G-cERP2-HOPXBarcoded piggybac transposon vector with Dox-inducible expression of HOPX
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192924pPB-cT3G-cERP2-FOSBarcoded piggybac transposon vector with Dox-inducible expression of FOS
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192925pPB-cT3G-cERP2-NR4A1Barcoded piggybac transposon vector with Dox-inducible expression of NR4A1
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192926pPB-cT3G-cERP2-RUNX2Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2
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192927pPB-cT3G-cERP2-TAF4BBarcoded piggybac transposon vector with Dox-inducible expression of TAF4B
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192928pPB-cT3G-cERP2-RUNX1Barcoded piggybac transposon vector with Dox-inducible expression of RUNX1
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Antibodies from Article