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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8426 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3200
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMouse Protein Kinase C theta
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Alt nameProtein Kinase C theta
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Alt namePKC theta
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SpeciesM. musculus (mouse)
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Insert Size (bp)2200
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Entrez GenePrkcq (a.k.a. A130035A12Rik, AW494342, PKC-0, PKC-theta, PKCtheta, Pkcq)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (unknown if destroyed)
- 3′ cloning site SmaI (unknown if destroyed)
- 5′ sequencing primer T7 ? (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS PKC theta was a gift from Frederic Mushinski (Addgene plasmid # 8426 ; http://n2t.net/addgene:8426 ; RRID:Addgene_8426) -
For your References section:
Unique expression pattern of protein kinase C-theta: high mRNA levels in normal mouse testes and in T-lymphocytic cells and neoplasms. Mischak H, Goodnight J, Henderson DW, Osada S, Ohno S, Mushinski JF. FEBS Lett 1993 Jul 12;326(1-3):51-5. 10.1016/0014-5793(93)81759-S PubMed 8325388