166,137 results

1. pMaster12

   Plasmid

   #58527

   Purpose

   Reprogram somatic cells to iPS cells

   Depositor

   Mario Capecchi

   Article

   Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

   Inserts

   neo
   tk
   oct4 (POU5F1 Human)
   Klf4 (KLF4 Human)
   Sox2 (SOX2 Human)
   Myc (MYC Human)
   EBNA-1
   Amp
   Lin28 (LIN28A Human)
   NANOG (NANOG Human)
   IRES
   NR5A2 (NR5A2 Human)
   miR302/367 (MIR367, MIR302A Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPR-EGFP

   Plasmid

   #75159

   Purpose

   Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti.PGK.chFP.W

   Plasmid

   #51008

   Purpose

   Lentivector that expresses chFP from internal human PGK promoter

   Depositor

   Rusty Lansford

   Insert

   mChFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   human PGK

   Available Since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. COC053

   Plasmid

   #209131

   Purpose

   Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.

   Depositor

   Christopher A. O'Callaghan

   Article

   O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. do

   Insert

   Enzyme BirA (birA E. coli)
   

   Expression

   Bacterial

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSF278

   Plasmid

   #167673

   Purpose

   Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBDC

   Plasmid

   #135188

   Purpose

   Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   CmR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Promoter

   cat

   Available Since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCVpuro-Bcl2

   Plasmid

   #191964

   Purpose

   Exogenous expression of Bcl2 WT

   Depositor

   Luca Scorrano

   Insert

   Bcl2 (BCL2 Human)
   

   Use

   Retroviral

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mRFP-Rab7

   Plasmid

   #14436

   Purpose

   Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

   Depositor

   Ari Helenius

   Article

   Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

   Insert

   Rab 7 (RAB7A Canis familialis)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   3 silent mutations near the C-terminus

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Noggin

   Plasmid

   #163586

   Purpose

   Expresses Noggin in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Nog (Nog Mouse)
   

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. HDAC4 Flag

    Plasmid

    #13821

    Purpose

    Mammalian expression of human histone deacetylase 4 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC4 (HDAC4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLCP FLAG B55 alpha

    Plasmid

    #13804

    Depositor

    William Hahn

    Article

    Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

    Insert

    PP2A B55 alpha Flag (PPP2R2A Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. GPS-MYC

    Plasmid

    #160130

    Purpose

    A reporter that monitors MYC protein expression.

    Depositor

    Channing Der

    Article

    Blake et al Sci Signal. 2019 Jul 16;12(590). pii: 12

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SPLICSFAST-LY-MT-P2A

    Plasmid

    #214897

    Purpose

    Expresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-LY-MT-P2A
    

    Tags

    none

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmTurquoise2-Mito

    Plasmid

    #36208

    Purpose

    In vivo visualization of the mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    MTS (COX8A Human)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. T7-TPase

    Plasmid

    #51818

    Purpose

    For in vitro transcription of Tol2 transposase using T7 Promoter

    Depositor

    Elly Tanaka

    Article

    Khattak et al Nat Protoc. 2014 Mar;9(3):529-40. doi: 1

    Insert

    Tol2 Transposase
    

    Use

    Unspecified

    Promoter

    T7

    Available Since

    April 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cterminal HA WDR59 pRK5

    Plasmid

    #46328

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR59 (WDR59 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti_CMV: Flag-Antares_SV40:PuroR

    Plasmid

    #183049

    Purpose

    Expresses FLAG-tagged Antares in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    Antares BRET reporter
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDestTol2pA2-U6:gRNA

    Plasmid

    #63157

    Purpose

    Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Type

    Empty backbone

    Use

    CRISPR; Tol2 destination vector for gateway

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-PIK3C2A

    Plasmid

    #161988

    Purpose

    Mammalian expression vector containing PIK3C2A tagged with GFP

    Depositor

    David Bryant, Volker Haucke

    Article

    Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

    Insert

    PIK3C2A (PIK3C2A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC18T-mini-Tn7T-Tp-ecfp

    Plasmid

    #65034

    Purpose

    TpR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    ecfp
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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